Sugarcane ScOPR1 gene enhances plant disease resistance through the modulation of hormonal signaling pathways
文献类型： 外文期刊
作者： Zou, Wenhui 1 ; Sun, Tingting 1 ; Chen, Yao 2 ; Wang, Dongjiao 2 ; You, Chuihuai 2 ; Zang, Shoujian 2 ; Lin, Peixia 2 ; Wu, Qibin 1 ; Su, Yachun 2 ; Que, Youxiong 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Natl Key Lab Trop Crop Breeding, Haikou 571101, Hainan, Peoples R China
2.Fujian Agr & Forestry Univ, Minist Agr & Rural Affairs, Coll Agr, Engn Res Ctr Sugarcane,Key Lab Sugarcane Biol & Ge, Fuzhou 350002, Fujian, Peoples R China
3.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China
关键词： Sugarcane; 12-Oxo-phytodienoic acid reductase; Pathogen infection; Genetic transformation; Resistance mechanism
期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）
ISSN： 0721-7714
年卷期： 2024 年 43 卷 6 期
页码：
收录情况： SCI
摘要： 12-Oxo-phytodienoate reductase (OPR) is an oxidoreductase that depends on flavin mononucleotide (FMN) and catalyzes the conversion of 12-oxophytodienoate (12-OPDA) into jasmonic acid (JA). It plays a key role in plant growth and development, and resistance to adverse stresses. In our previous study, we have obtained an OPR gene (ScOPR1, GenBank Accession Number: MG755745) from sugarcane. This gene showed positive responses to methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), and Sporisorium scitamineum, suggesting its potential for pathogen resistance. Here, in our study, we observed that Nicotiana benthamiana leaves transiently overexpressing ScOPR1 exhibited weaker disease symptoms, darker 3,3-diaminobenzidine (DAB) staining, higher accumulation of reactive oxygen species (ROS), and higher expression of hypersensitive response (HR) and SA pathway-related genes after inoculation with Ralstonia solanacearum and Fusarium solanacearum var. coeruleum. Furthermore, the transgenic N. benthamiana plants stably overexpressing the ScOPR1 gene showed enhanced resistance to pathogen infection by increasing the accumulation of JA, SA, and glutathione S-transferase (GST), as well as up-regulating genes related to HR, JA, SA, and ROS signaling pathways. Transcriptome analysis revealed that the specific differentially expressed genes (DEGs) in ScOPR1-OE were significantly enriched in hormone transduction signaling and plant-pathogen interaction pathways. Finally, a functional mechanism model of the ScOPR1 gene in response to pathogen infection was depicted. This study provides insights into the molecular mechanism of ScOPR1 and presents compelling evidence supporting its positive involvement in enhancing plant disease resistance.
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