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Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers

文献类型： 外文期刊

作者： Zou, Zhi 1 ; Zheng, Yujiao 1 ; Zhang, Zhongtian 1 ; Xiao, Yanhua 1 ; Xie, Zhengnan 1 ; Chang, Lili 1 ; Zhang, Li 1 ; Zhao, Yongguo 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Key Lab Biosafety Monitoring & Mol Breedin, Inst Trop Biosci & Biotechnol, Sanya Res Inst, Haikou 571101, Hainan, Peoples R China

2.South Cent Minzu Univ, Coll Life Sci, Hubei Prov Key Lab Protect & Applicat Special Pla, Wuhan 430074, Hubei, Peoples R China

3.Guangdong Univ Petrochem Technol, Maoming 525000, Guangdong, Peoples R China

关键词： Oil crop; Oil accumulation; Expression divergence; Orthogroup; Protein interaction; Synteny analysis

期刊名称：PLANT CELL REPORTS （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2023 年 42 卷 11 期

页码：

收录情况： SCI

摘要： Oleosins (OLEs) are abundant structural proteins of lipid droplets (LDs) that function in LD formation and stabilization in seeds of oil crops. However, little information is available on their roles in vegetative tissues. In this study, we present the first genome-wide characterization of the oleosin family in tigernut (Cyperus esculentus L., Cyperaceae), a rare example accumulating high amounts of oil in underground tubers. Six members identified represent three previously defined clades (i.e. U, SL and SH) or six out of seven orthogroups (i.e. U, SL1, SL2, and SH1-3) proposed in this study. Comparative genomics analysis reveals that lineage-specific expansion of Clades SL and SH was contributed by whole-genome duplication and dispersed duplication, respectively. Moreover, presence of SL2 and SH3 in Juncus effuses implies their appearance sometime before Cyperaceae-Juncaceae divergence, whereas SH2 appears to be Cyperaceae specific. Expression analysis showed that CeOLE genes exhibit a tuber-predominant expression pattern and transcript levels are considerably more abundant than homologs in the close relative Cyperus rotundus. Moreover, CeOLE mRNA and protein abundances were shown to positively correlate with oil accumulation during tuber development. Additionally, two dominant isoforms (i.e. CeOLE2 and -5) were shown to locate in LDs as well as the endoplasmic reticulum of tobacco (Nicotiana benthamiana) leaves, and are more likely to function in homo and heteromultimers. Furthermore, overexpression of CeOLE2 and -5 in tobacco leaves could significantly increase the oil content, supporting their roles in oil accumulation. These findings provide insights into lineage-specific family evolution and putative roles of CeOLE genes in oil accumulation of vegetative tissues, which facilitate further genetic improvement for tigernut.

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[1]Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers. Zou, Zhi,Zheng, Yujiao,Zhang, Zhongtian,Xiao, Yanhua,Xie, Zhengnan,Chang, Lili,Zhang, Li,Zhao, Yongguo,Zhang, Li,Zhao, Yongguo. 2023

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