文献类型： 外文期刊

作者： Yang, Jing ¹ ; Li, Juan ⁴ ; Sun, Ju ¹ ; Li, Jiaming ¹ ; Fu, Guanghua ⁷ ; Tian, Tian ¹ ; Yang, Yongchun ⁸ ; Lu, Xuancheng ¹⁰ ; Li, Shan ⁴ ; Wang, Lixia ¹ ; Dong, Jia ¹ ; Wu, Mingjia ¹ ; Liu, Yun ¹ ; Li, Delong ¹¹ ; Hu, Dongfang ¹² ; Dong, Hui ⁴ ; Shang, Ruoyu ¹ ; Wang, Yanqing ¹ ; Yuan, Kunpeng ¹ ; Ran, Lin ¹ ; Sun, Honglei ¹³ ; Tian, Wenxia ⁶ ; Huang, Yu ⁷ ; Liu, Jinhua ¹³ ; Liu, Wenjun ¹ ; Shi, Weifeng ¹⁴ ; Gao, George F. ¹ ; Bi, Yuhai ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, Ctr Influenza Res & Early Warning CASCIRE,Lab Pat, CAS TWAS Ctr Excellence Emerging Infect Dis CEEID, Beijing, Peoples R China

2.Univ Chinese Acad Sci, Beijing, Peoples R China

3.Beijing Res Ctr Resp Infect Dis, Beijing Key Lab Surveillance Early Warning & Path, Beijing, Peoples R China

4.Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Emerging Infect Dis Univ Shandong, Jinan, Peoples R China

5.Shandong First Med Univ & Shandong Acad Med Sci, Sch Clin & Basic Med Sci, Jinan, Peoples R China

6.Shanxi Agr Univ, Coll Vet Med, Jinzhong, Peoples R China

7.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou, Peoples R China

8.Zhejiang A&F Univ, Coll Anim Sci & Technol, Zhejiang Prov Engn Lab Anim Hlth & Internet Techn, Hangzhou, Peoples R China

9.Zhejiang A&F Univ, Coll Vet Med, Hangzhou, Peoples R China

10.Chinese Ctr Dis Control & Prevent China CDC, Lab Anim Ctr, Natl Key Lab Intelligent Tracking & Forecasting I, Beijing, Peoples R China

11.Southwest Univ, Coll Vet Med, Chongqing, Peoples R China

12.Henan Inst Sci & Technol, Coll Anim Sci & Technol, Xinxiang, Peoples R China

13.China Agr Univ, Coll Vet Med, Natl Key Lab Vet Publ Hlth & Safety, Key Lab Prevent & Control Avian Influenza & Other, Beijing, Peoples R China

14.Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Infect Dis, Sch Med, Shanghai, Peoples R China

15.Shanghai Jiao Tong Univ, Shanghai Inst Virol, Sch Med, Shanghai, Peoples R China

16.Zhejiang Univ, DH Chen Sch Universal Hlth, Hangzhou, Peoples R China

17.Jinzhou Med Univ, Collaborat Innovat Ctr Prevent & Control Zoonoses, Jinzhou, Peoples R China

期刊名称：NATURE MICROBIOLOGY （ 影响因子：19.4； 五年影响因子：20.7 ）

ISSN： 2058-5276

年卷期： 2025 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Nationwide surveillance of avian influenza viruses (AIVs) in live poultry markets across China has occurred since 2014, providing a resource for AIV prevalence and genetic diversity studies. Here we report that 3,237 of 18,425 samples from poultry were AIV positive (17.57%) between 2019 and 2023, with H9N2 being the dominant subtype. We developed an automated phylogeny-based nomenclature system to classify genetic clades of the dominant H9N2 lineage, the BJ94 lineage. Using this model, we found that ten haemagglutinin (HA) sub-subclades cocirculated in poultry and showed antigenic variation. In addition, 99.46% and 96.17% of H9N2 AIVs in 2021-2023 possessed human-receptor binding-related HA-L226 and human MxA-resistance-related NP-N52 mutations, respectively. H9N2 strains with these two mutations preferred human-type receptors and increased replication in human cells in vitro, regardless of the presence of PB2-V/K/E627. Moreover, H9N2 AIVs containing HA-L226, PB2-V/K627 and NP-N52 were transmitted from infected to naive guinea pigs and ferrets through direct contact and respiratory droplet. This highlights the potential zoonotic risks of H9N2 AIVs.