Identification and molecular characterization of two novel viruses from Allamanda cathartica in China
文献类型： 外文期刊
作者： Che, Haiyan 1 ; Ma, Yuxin 1 ; Wu, Yangxin 3 ; Long, Haibo 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Peoples R China
2.Hainan Key Lab Monitoring & Control Trop Agr Pests, Haikou 571101, Peoples R China
3.Hainan Univ, Sch Life & Hlth Sci, Haikou 570228, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.7； 五年影响因子：2.4 ）
ISSN： 0304-8608
年卷期： 2024 年 169 卷 6 期
页码：
收录情况： SCI
摘要： Allamanda cathartica is an ornamental medicinal plant that grows widely in the tropics. In the present study, two novel viruses, Allamanda chlorotic virus A (AlCVA) and Allamanda chlorotic virus B (AlCVB), were identified in an A. cathartica plant with interveinal chlorosis by ribosomal RNA-depleted total-RNA sequencing. Phylogenetic analysis and sequence comparisons confirmed that AlCVA and AlCVB belong to the families Closteroviridae and Betaflexiviridae, respectively. Long, flexuous, filamentous virus particles approximately 12 nm in diameter and 784-2291 nm in length were observed using transmission electron microscopy. A specific RT-PCR assay was used to demonstrate a consistent association of viral infection with symptoms.
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