Functional analysis of StWRKY75 gene in response to heat stress in potato (Solanum tuberosum L.)
文献类型： 外文期刊
作者： Zhu, Xi 1 ; Duan, Xiaoqin 4 ; Majeed, Yasir 4 ; Luo, Junfu 1 ; Guan, Nengkang 1 ; Zheng, Haifei 1 ; Zou, Huafen 1 ; Jin, Hui 1 ; Chen, Zhuo 1 ; Zhang, Yu 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Trop Fruit Biol, Minist Agr & Rural Affairs, Zhanjiang, Guangdong, Peoples R China
2.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Key Lab Hainan Prov Postharvest Physiol & Technol, Zhanjiang, Guangdong, Peoples R China
3.Chinese Acad Trop Agr Sci, Sanya Res Inst, Natl Key Lab Trop Crop Breeding, Sanya, Peoples R China
4.Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou, Peoples R China
5.Gansu Agr Univ, Coll Agron, Lanzhou, Peoples R China
6.Yunnan Agr Univ, Coll Trop Crops, Puer, Peoples R China
关键词：
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2025 年 16 卷
页码：
收录情况： SCI
摘要： Heat stress severely restricts potato tuber growth and development, yet the roles of WRKY transcription factors (TFs) in mediating heat responses remain poorly understood. Using quantitative reverse transcription PCR (qRT-PCR), we identified StWRKY75 in potato cultivars 'Atlantic' and 'Desiree' as a heat-inducible gene, showing significant upregulation under 30 degrees C and 35 degrees C treatments. Phylogenetic analysis classified StWRKY75 into the WRKY Group II family, with close evolutionary homology to tomato SlWRKY75, and subcellular localization confirmed its nuclear targeting. To characterize its function, we generated transgenic lines overexpressing StWRKY75 (OE) or knockdown by RNA interference (RNAi). Under heat stress conditions, OE plants demonstrated superior thermotolerance compared to non-transgenic (NT) controls, manifested by improved growth parameters (plant height, tuber weight per plant, fresh weight, dry weight, root fresh weight, and root dry weight), enhanced activities of antioxidant enzymes (ascorbate peroxidase [APX], catalase [CAT], peroxidase [POD], and superoxide dismutase [SOD]), increased proline accumulation, elevated photosynthetic efficiency, and reduced malondialdehyde (MDA) content. Conversely, RNAi lines exhibited compromised thermotolerance with reversed growth parameters and biochemical characteristics. qRT-PCR analysis revealed that StWRKY75 positively regulated key heat-stress responsive genes, including those encoding antioxidant enzymes (StAPX, StCAT, StSOD, StPOD), proline biosynthesis Pyrroline-5-carboxylate synthase (StP5CS), and heat shock proteins (StHSP20, StHSP70, StHSP90). These findings demonstrate that StWRKY75 acts as a positive regulator of potato thermotolerance by enhancing growth, antioxidant capacity, proline metabolism, photosynthesis, and heat-responsive gene expression, providing a valuable target for improving crop heat resilience in breeding programs.
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