您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Co-expression of transcription factors ZmC1 and ZmR2 establishes an efficient and accurate haploid embryo identification system in maize

文献类型： 外文期刊

作者： Chen, Chen 1 ; Liu, Xiaoqing 3 ; Li, Suzhen 3 ; Liu, Chenxu 1 ; Zhang, Yuling 1 ; Luo, Lili 3 ; Miao, Liqing 3 ; Yang, Wenzhu 3 ; Xiao, Zijian 1 ; Zhong, Yu 1 ; Li, Jiansheng 1 ; Chen, Rumei 3 ; Chen, Shaojiang 1 ;

作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Maize Res Inst, Beijing 100097, Peoples R China

3.Chinese Acad Agr Sci, Crop Funct Genome Res Ctr, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： anthocyanin marker; doubled haploid (DH); haploid identification; immature haploid embryos; maize (Zea mays L; )

期刊名称：PLANT JOURNAL （ 影响因子：7.091； 五年影响因子：8.028 ）

ISSN： 0960-7412

年卷期：

页码：

收录情况： SCI

摘要： Because of their high efficiency during chromosome doubling, immature haploid maize (Zea mays L.) embryos are useful for doubled haploid production. The R1-nj marker is commonly used in doubled haploid breeding and has improved the efficiency of haploid identification. However, its effectiveness is limited by genetic background and environmental factors. We addressed this technical challenge by developing an efficient and accurate haploid embryo identification marker through co-expression of two transcription factor genes (ZmC1 and ZmR2) driven by the embryo-aleurone-specific bidirectional promoter P-ZmBD1; these factors can activate anthocyanin biosynthesis in the embryo and aleurone layer during early seed development. We developed a new haploid inducer, Maize Anthocyanin Gene InduCer 1 (MAGIC1), by introducing the transgenes into the haploid inducer line CAU6. MAGIC1 could identify haploids at 12 days after pollination, which is nine days earlier than CAU6. Importantly, MAGIC1 increased haploid identification accuracy to 99.1%, compared with 88.3% for CAU6. In addition, MAGIC1 could effectively overcome the inhibition of anthocyanin synthesis in some germplasms. Furthermore, an upgraded anthocyanin marker was developed from ZmC1 and ZmR2 to generate MAGIC2, which could identify haploids from diploids due to differential anthocyanin accumulation in immature embryos, coleoptiles, sheaths, roots, leaves, and dry seeds. This haploid identification system is more efficient and accurate than the conventional R1-nj-based method, and it simplifies the haploid identification process. Therefore, this system provides technical support for large-scale doubled haploid line production.

  • 相关文献

[1]Co-expression of transcription factors ZmC1 and ZmR2 establishes an efficient and accurate haploid embryo identification system in maize. Chen, Chen,Liu, Chenxu,Zhang, Yuling,Xiao, Zijian,Zhong, Yu,Li, Jiansheng,Chen, Shaojiang,Chen, Chen,Liu, Xiaoqing,Li, Suzhen,Luo, Lili,Miao, Liqing,Yang, Wenzhu,Chen, Rumei.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台