Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells
文献类型： 外文期刊
作者： Pan, Zaozao 1 ; Huang, Jiaqiang 2 ; Hu, Ting 1 ; Zhang, Yonghong 1 ; Zhang, Lingyu 1 ; Zhang, Jiaxi 1 ; Cui, Defeng 1 ; Li, Lu 3 ; Wang, Jing 3 ; Wu, Qiong 1 ;
作者机构： 1.Beijing Univ Agr, Anim Sci & Technol Coll, Beijing 100096, Peoples R China
2.China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Dept Nutr & Hlth, Beijing 100193, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China
关键词： Bisphenol A; selenium nanoparticles; tight junction; oxidative stress; apoptosis; endoplasmic reticulum stress
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 8 期
页码：
收录情况： SCI
摘要： Bisphenol A (BPA) is widely used to harden plastics and polycarbonates and causes serious toxic effects in multiple organs, including the intestines. Selenium, as an essential nutrient element for humans and animals, exhibits a predominant effect in various physiological processes. Selenium nanoparticles have attracted more and more attention due to their outstanding biological activity and biosafety. We prepared chitosan-coated selenium nanoparticles (SeNPs) and further compared the protective effects, and investigated the underlying mechanism of SeNPs and inorganic selenium (Na2SeO3) on BPA-induced toxicity in porcine intestinal epithelial cells (IPEC-J2). The particle size, zeta potential, and microstructure of SeNPs were detected by using a nano-selenium particle size meter and a transmission electron microscope. IPEC-J2 cells were exposed to BPA alone or simultaneously exposed to BPA and SeNPs or Na2SeO3. The CCK8 assay was performed to screen the optimal concentration of BPA exposure and the optimal concentration of SeNPs and Na2SeO3 treatment. The apoptosis rate was detected by flow cytometry. Real-time PCR and Western blot methods were used to analyze the mRNA and protein expression of factors related to tight junctions, apoptosis, inflammatory responses and endoplasmic reticulum stress. Increased death and morphological damage were observed after BPA exposure, and these increases were attenuated by SeNPs and Na2SeO3 treatment. BPA exposure disturbed the tight junction function involved with decreased expression of tight junction protein Zonula occludens 1 (ZO-1), occludin, and claudin-1 proteins. Proinflammatory response mediated by the transcription factor nuclear factor-k-gene binding (NF-kappa B), such as elevated levels of interleukin-1 beta(IL-1 beta), interleukin-6 (IL-6), interferon-gamma (IFN-gamma), interleukin-17 (IL-17), and tumor necrosis factor-alpha (TNF-alpha) expression was induced at 6 and 24 h after BPA exposure. BPA exposure also disturbed the oxidant/antioxidant status and led to oxidative stress. IPEC-J2 cell apoptosis was induced by BPA exposure, as indicated by increased BCL-2-associated X protein (Bax), caspase 3, caspase 8, and caspase 9 expression and decreased B-cell lymphoma-2 (Bcl-2) and Bcl-xl expression. BPA exposure activated the endoplasmic reticulum stress (ERS) mediated by the receptor protein kinase receptor-like endoplasmic reticulum kinase (PERK), Inositol requiring enzyme 1 (IRE1 alpha), and activating transcription factor 6 (ATF6). We found that treatment with SeNPs and Na2SeO3 can alleviate the intestinal damage caused by BPA. SeNPs were superior to Na2SeO3 and counteracted BPA-induced tight junction function injury, proinflammatory response, oxidative stress, apoptosis, and ERS stress. Our findings suggest that SeNPs protect intestinal epithelial cells from BPA-induced damage, partly through inhibiting ER stress activation and subsequently attenuating proinflammatory responses and oxidative stress and suppressing apoptosis, thus enhancing the intestinal epithelial barrier function. Our data indicate that selenium nanoparticles may represent an effective and reliable tool for preventing BPA toxicity in animals and humans.
- 相关文献
作者其他论文 更多>>
-
EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research
作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang
关键词：metagenome; microbiome; microbiota; pipeline; visualization
-
Calycosin, a Bioactive Isoflavone, Ameliorates Oxidative Stress and Inflammation in Lipopolysaccharide-Induced Intestinal Cell Damage Model via the Nrf2 and NF-κB Signaling Pathways
作者：Li, Lu;Che, Yuyan;Zhu, Longlong;Liu, Zeqing;Song, Yanchen;Chen, Meixia;Wang, Jing;Li, Lu;Che, Yuyan;Song, Yanchen;Zhu, Longlong;Liu, Zeqing;Wu, Qiong;Chen, Meixia;Wang, Jing
关键词：calycosin; cell damage; inflammation; LPS; NF-kappa B pathway; Nrf2 pathway; oxidative stress
-
Chromosome-level genome assembly of tetraploid Chinese cherry (Prunus pseudocerasus)
作者：Zhang, Wei;Wang, Xuncheng;Peng, Junbo;Xing, Qikai;Yan, Jiye;Wang, Jing;Duan, Xuwei;Zhang, Xiaoming;Zhang, Kaichun
关键词：
-
New insights into the cold tolerance of upland switchgrass by integrating a haplotype-resolved genome and multi-omics analysis
作者：Wu, Bingchao;Luo, Dan;Yan, Haidong;Ma, Xixi;Zhu, Jie;Jia, Jiyuan;Sun, Min;Xie, Zheni;Wang, Xiaoshan;Huang, Linkai;Yue, Yuesen;He, Min;Zhao, Bingyu;Xu, Bin;Wang, Jing;Sun, Min
关键词：Upland switchgrass; Haplotype-resolved genomes; Allele-specific expression genes; Cold tolerance
-
Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry
作者：Feng, Chen;Guo, Qingqing;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Feng, Chen;Wu, Chuanbao;Wang, Jing;Zhang, Xiaoming;Yan, Guohua;Zhou, Yu;Wang, Wei;Zhang, Kaichun;Duan, Xuwei;Guo, Qingqing;Xue, Zheyong
关键词：Sweet cherry; UDP-glycosyltransferase; PavUGT48; Anthocyanin; Amygdalin
-
Effects of different rootstocks on fruit quality and non-volatile flavor-related compounds of sweet cherry 'summit'
作者：Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Wang, Naiyu;Guo, Qingqing;Li, Tianzhong;Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Wang, Naiyu;Zhang, Xiaoming;Guo, Qingqing;Yan, Guohua;Wang, Jing;Wu, Chuanbao;Zhou, Yu;Zhou, Junman;Zhang, Kaichun;Duan, Xuwei;Zhou, Junman
关键词：Prunus avium; Fertilization; Grafting; Sugar; Organic acid; Phenolic acid
-
Population sequencing of cherry accessions unravels the evolution of Cerasus species and the selection of genetic characteristics in edible cherries (vol 5, 6, 2025)
作者：Lei, Yahui;Jiu, Songtao;Xu, Yan;Lv, Zhengxin;Liu, Xunju;Wang, Lei;Wang, Li;Wang, Jiyuan;Zhang, Zhuo;Wang, Shiping;Zhang, Caixi;Lei, Yahui;Dong, Xiao;Dong, Yang;Chen, Baozheng;Dong, Yang;Bernard, Anthony;Barreneche, Teresa;Elisabeth, Dirlewanger;Cai, Yuliang;Zheng, Wei;Zhang, Xu;Li, Fangdong;Li, Hongwen;Liu, Congli;Li, Ming;Wang, Jing;Zhu, Jijun;Peng, Lei;Yu, Fei;Duan, Shengchang
关键词：
