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Heat shock factor ZmHsf17 positively regulates phosphatidic acid phosphohydrolase ZmPAH1 and enhances maize thermotolerance

文献类型： 外文期刊

作者： Zhang, Huaning 1 ; Meng, Xiangzhao 1 ; Liu, Ran 1 ; Li, Ran 2 ; Wang, Yantao 1 ; Ma, Zhenyu 1 ; Liu, Zihui 1 ; Duan, Shuonan 1 ; Li, Guoliang 1 ; Guo, Xiulin 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Biotechnol & Food Sci, Shijiazhuang 050051, Peoples R China

2.Hebei North Univ, Coll Agr & Forestry Sci & Technol, Zhangjiakou 075000, Peoples R China

3.Hebei Univ Engn, Sch Landscape & Ecol Engn, Handan 056000, Peoples R China

关键词： Heat shock factor; maize; membrane lipid component; thermotolerance; transcriptional regulation; ZmPAH1

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：5.7； 五年影响因子：6.8 ）

ISSN： 0022-0957

年卷期： 2024 年 76 卷 2 期

页码：

收录情况： SCI

摘要： Heat stress adversely impacts plant growth, development, and grain yield. Heat shock factors (Hsf), especially the HsfA2 subclass, play a pivotal role in the transcriptional regulation of genes in response to heat stress. In this study, the coding sequence of maize ZmHsf17 was cloned. ZmHsf17 contained conserved domains including a DNA binding domain, oligomerization domain, and transcriptional activation domain. The protein was nuclear localized and had transcription activation activity. Yeast two-hybrid and split luciferase complementation assays confirmed the interaction of ZmHsf17 with members of the maize HsfA2 subclass. Overexpression of ZmHsf17 in maize significantly increased chlorophyll content and net photosynthetic rate, and enhanced the stability of cellular membranes. Through integrative analysis of ChIP-seq and RNA-seq datasets, ZmPAH1, encoding phosphatidic acid phosphohydrolase of lipid metabolic pathways, was identified as a target gene of ZmHsf17. The promoter fragment of ZmPAH1 was bound by ZmHsf17 in protein-DNA interaction experiments in vivo and in vitro. Lipidomic data also indicated that the overexpression of ZmHsf17 increased levels of some critical membrane lipid components of maize leaves under heat stress. This research provides new insights into the role of the ZmHsf17-ZmPAH1 module in regulating thermotolerance in maize.

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