文献类型： 外文期刊

作者： Liu, Baocai ¹ ; Chen, Jingying ² ; Zhang, Wujun ² ; Huang, Yingzhen ² ; Zhao, Yunqing ² ; Juneidi, Seifu ⁶ ; Dekebo, Aman ⁷ ; Wang, Meijuan ⁹ ; Shi, Le ¹ ; Hu, Xuebo ¹ ;

作者机构： 1.Huazhong Agr Univ, Inst Med Plants, Coll Plant Sci & Technol, Wuhan, Peoples R China

2.Fujian Acad Agr Sci, Inst Agr Bioresource, Fuzhou, Peoples R China

3.Huazhong Agr Univ, Innovat Acad Int Tradit Chinese Med Mat, Wuhan, Peoples R China

4.Huazhong Agr Univ, Natl Reg Joint Engn Res Ctr Hubei Med Plant Breed, Wuhan, Peoples R China

5.Huazhong Agr Univ, Med Plant Engn Res Ctr Hubei Prov, Wuhan, Peoples R China

6.Adama Sci & Technol Univ, Sch Nat Sci, Dept Appl Biol, Adama, Ethiopia

7.Adama Sci & Technol Univ, Sch Appl Nat Sci, Appl Chem Dept, Adama, Ethiopia

8.Adama Sci & Technol Univ, Inst Pharmaceut Sci, Adama, Ethiopia

9.Shengnongjia Acad Forestry, Shengnongjia, Hubei, Peoples R China

关键词： Pholidota chinensis; gastrodin; metabolome; transcriptome; molecular mechanism

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Pholidota chinensis Lindl. is an epiphytic or lithophytic perennial herb of Orchidaceae family used as a garden flower or medicinal plant to treat high blood pressure, dizziness and headache in traditional Chinese medicine. Gastrodin (GAS) is considered as a main bioactive ingredient of this herb but the biosynthetic pathway remains unclear in P. chinensis. To elucidate the GAS biosynthesis and identify the related genes in P. chinensis, a comprehensive analysis of transcriptome and metabolome of roots, rhizomes, pseudobulbs and leaves were performed by using PacBio SMART, Illumina Hiseq and Ultra Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC-MS/MS). A total of 1,156 metabolites were identified by UPLC-MS/MS, of which 345 differential metabolites were mainly enriched in phenylpropanoid/phenylalanine, flavone and flavonol biosynthesis. The pseudobulbs make up nearly half of the fresh weight of the whole plant, and the GAS content in the pseudobulbs was also the highest in four tissues. Up to 23,105 Unigenes were obtained and 22,029 transcripts were annotated in the transcriptome analysis. Compared to roots, 7,787, 8,376 and 9,146 differentially expressed genes (DEGs) were identified in rhizomes, pseudobulbs and leaves, respectively. And in total, 80 Unigenes encoding eight key enzymes for GAS biosynthesis, were identified. Particularly, glycosyltransferase, the key enzyme of the last step in the GAS biosynthetic pathway had 39 Unigenes candidates, of which, transcript28360/f2p0/1592, was putatively identified as the most likely candidate based on analysis of co-expression, phylogenetic analysis, and homologous searching. The metabolomics and transcriptomics of pseudobulbs versus roots showed that 8,376 DEGs and 345 DEMs had a substantial association based on the Pearson's correlation. This study notably enriched the metabolomic and transcriptomic data of P. chinensis, and it provides valuable information for GAS biosynthesis in the plant.