文献类型： 外文期刊

作者： Yi, Ming ¹ ; Lou, Jinxiu ² ; Zhu, Wanlu ³ ; Li, Dan ⁴ ; Yu, Pingfeng ³ ; Lu, Huijie ³ ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Univ Technol, Coll Environm, Hangzhou 310014, Peoples R China

3.Zhejiang Univ, Coll Environm & Resource Sci, Key Lab Environm Remediat & Ecol Hlth, Minist Educ, Hangzhou 310058, Peoples R China

4.Fudan Univ, Shanghai Key Lab Atmospher Particle Pollut & Prev, Dept Environm Sci & Engn, Shanghai 200438, Peoples R China

5.Zhejiang Univ, Acad Ecol Civilizat, Hangzhou 310058, Peoples R China

关键词： -blocker; Activated sludge; Degradation pathway; Metoprolol acid; TfdA

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2023 年 444 卷

页码：

收录情况： SCI

摘要： The recalcitrant 8-blockers have been widely detected in aquatic environments up to several hundred ??g/L, which are major contributors to 81 antagonistic activities in wastewater. Their biodegradation mechanisms remain obscure, hindering the development of efficient removal techniques. This study constructed the biodegradation pathways for three typical 8-blockers, namely atenolol, metoprolol, and propranolol, assessed the toxicity of their major biotransformation products, and identified the key enzyme catalyzing the O-dealkylation reaction leading to pollutant mineralization. Atenolol and metoprolol degradation was more efficient than that of propranolol by activated sludge, producing metoprolol acid (MTPA) as a major intermediate. Hydrogenophaga sp. YM1 isolated from activated sludge possess the a-ketoglutarate dependent dioxygenase (TfdA) responsible for O- dealkylation of MTPA and propranolol, producing 4-hydroxyphenylacetic acid (4-HPA) that can be further degraded and ultimately enters the TCA cycle. The role of TfdA was verified by proteomics, enzyme stimulation/ inhibition tests, and gene knockout experiments. Molecular docking suggests its different interactions with MTPA and propranolol. Acetate facilitated the degradation of 8-blockers efficiently. The results may shed light on enhanced biological removals of broader 8-blockers and their transformation products in the environment.