In vivo assembly in tobacco cells to elucidate and engineer the biosynthesis of 4-hydroxydihydrocinnamaldehyde from Gloriosa superba
文献类型： 外文期刊
作者： Xiong, Zhiqiang 1 ; Ding, Zhuoying 1 ; Sun, Jingyi 1 ; Jiang, Xuefei 3 ; Cong, Hanqing 1 ; Sun, Huapeng 1 ; Qiao, Fei 1 ;
作者机构： 1.Natl Key Lab Trop Crops Breeding, Sanya 572024, Peoples R China
2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab Crop Gene Resources & Germplasm Enhancemen, Minist Agr, Haikou 571101, Peoples R China
3.Hainan Univ, Sanya Nanfan Res Inst, Coll Hort, Hainan Key Lab Sustainable Utilizat Trop Bioresour, Haikou 570228, Peoples R China
关键词： 4-HDCA; Biosynthesis pathway; Heterologous reconstruction; Tobacco cells; Stable transformation
期刊名称：PLANT CELL REPORTS （ 影响因子：4.5； 五年影响因子：6.1 ）
ISSN： 0721-7714
年卷期： 2024 年 43 卷 10 期
页码：
收录情况： SCI
摘要：
The precursor, 4-hydroxydihydrocinnamaldehyde (4-HDCA), participates in the biosynthesis of the carbon skeleton of colchicine, which is derived from L-phenylalanine. However, one hypothesis proposed that 4-HDCA is synthesized by sharing the early part of the monolignol pathway in G. superba. In this study, we validated this prediction and identified the enzymatic functions involved in this pathway. GsDBR1 is a crucial enzyme to illustrate 4-HDCA diverging from monolignol pathway, we first confirmed its reductase activity on 4-coumaraldehyde, an important intermediate compound in monolignol biosynthesis. Then, the biochemical function of recombinant enzymes belonging to the other four families were verified to elucidate the entire process of 4-HDCA biosynthesis from L-phenylalanine. After reconstruction, the 4-HDCA was 78.4 ng/g with fresh weight (FW) of transgenic tobacco cells, and the yield increased to 168.22 ng/g
- 相关文献
作者其他论文 更多>>
-
Transcriptome and metabolome analysis of low-temperature storage on fruit quality deterioration of mangoes
作者：Cong, Hanqing;Huo, Ting;Chen, Yeyuan;Bai, Yibo;Luo, Haiyan;Fu, Xiaona;Wang, Rongxiang;Qiao, Fei;Cong, Hanqing;Huo, Ting;Chen, Yeyuan;Bai, Yibo;Luo, Haiyan;Fu, Xiaona;Wang, Rongxiang;Qiao, Fei;Huo, Ting;Huang, Xinran;Chen, Yeyuan;Qiao, Fei;Seleiman, Mahmoud F.;Ahmad, Tanveer;Guan, Pingyin;Bashir, Mohsin;Asif, Muhammad
关键词：Mango; low temperature storage; volatile organic compounds; metabolome; transcriptome
-
Engineering and Optimization of the Salidroside Production in Tobacco Cells by Reconstructed Biosynthetic Genes from Rhodiola rosea
作者：Sun, Huapeng;Tang, Xin;Zhang, Yingying;Fu, Xiaona;Wang, Rongxiang;Shahzaib, Muhammad;Qiao, Fei
关键词：salidroside; reconstruction; heterologous production; tobacco cells; endogenous
l -tyrosine -
Enhancing salt tolerance and yield potential in cotton: insights from physiological responses, genetic variability, and heterosis
作者：Zafar, Muhammad Mubashar;Jiang, Xuefei;Zafar, Muhammad Mubashar;Zahid, Muhammad;Razzaq, Abdul;Anwar, Zunaira;Ijaz, Aqsa;Iqbal, Muhammad Shahid;Farid, Ghulam;Seleiman, Mahmoud F.;Zaman, Rana Qammar Uz;Rauf, Abdur
关键词：Cotton; salt stress; line x tester; general combining ability (GCA); specific combining ability (SCA); heterosis
-
Identification of a missing Pictet-Spenglerase in the Gloriosa superba L. colchicine biosynthesis pathway
作者：Sun, Jingyi;Sun, Huapeng;Xiong, Zhiqiang;Fu, Xiaona;Cong, Hanqing;Qiao, Fei;Sun, Jingyi;Sun, Huapeng;Xiong, Zhiqiang;Fu, Xiaona;Cong, Hanqing;Qiao, Fei;Sun, Jingyi;Xiong, Zhiqiang;Jiang, Xuefei
关键词：Pictet-Spenglerases; 1-phenethylisoquinoline scaffold; Colchicine biosynthesis;
Gloriosa superba L. -
The role of nitric oxide and nitrogen in mediating copper stress in Brassica juncea L.
作者：Rather, Bilal A.;Masood, Asim;Khan, Nafees A.;Rather, Bilal A.;Jiang, Xuefei;Zafar, Muhammad Mubashar;Qiao, Fei;Cong, Hanqing
关键词：Copper stress; Mustard; Nitric oxide; Nitrogen; Photosynthesis and antioxidant system
-
Sandalwood Essential Oil (SEO) Readily Inhibits Colletotrichum gloeosporioides-Mediated Anthracnose in Post-Harvest Stored Mango (Mangifera indica L. cv. 'Keitt')
作者：Shahzaib, Muhammad;Wang, Shenzhen;Yao, Rundong;Wang, Rongxiang;Fu, Xiaona;Cong, Hanqing;Zafar, Muhammad Mubashar;Qiao, Fei;Shahzaib, Muhammad;Wang, Shenzhen;Yao, Rundong;Wang, Rongxiang;Fu, Xiaona;Cong, Hanqing;Zafar, Muhammad Mubashar;Qiao, Fei;Shahzaib, Muhammad;Jiang, Xuefei;Zafar, Muhammad Mubashar;Wang, Shenzhen;Guan, Pingyin;Zhu, Yuandi;Ercisli, Sezai;Zafar, Muhammad Mubashar;Qiao, Fei;Zafar, Muhammad Mubashar;Qiao, Fei;Zafar, Muhammad Mubashar
关键词：Essential oils; sandalwood essential oil; Colletotrichum gloeosporioides; anthracnose; fungitoxic compounds
-
Electron beam irradiation significantly improves post-harvest fruit quality and shelf-life of Mango (Mangifera indica L.)
作者：Zheng, Bo;Shahzaib, Muhammad;Mi, Ziqing;Cong, Hanqing;Qiao, Fei;Zheng, Bo;Wang, Yerong;Xu, Xiaoling;Liu, Caihua;Zheng, Bo;Mi, Ziqing;Zhu, Yuandi;Shahzaib, Muhammad;Jiang, Xuefei;Shahzaib, Muhammad;Jiang, Xuefei;Qiao, Fei
关键词：Electron beam irradiation; Post-harvest anthracnose; Mango transcriptome; Ripening delay; Antioxidant enzymes
