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Identification of miRNAs affecting anthocyanin accumulation in different varieties of potato based on transcriptome analysis

文献类型： 外文期刊

作者： Qi, Enfang 1 ; Jia, Xiaoxia 1 ; Huang, Wei 1 ; Wen, Guohong 1 ; Lv, Heping 1 ; Li, Jianwu 1 ; Li, Zhang 1 ;

作者机构： 1.Gansu Acad Agr Sci, Potato Res Inst, Nongkeyuan Xincun 1, Lanzhou 730070, Gansu, Peoples R China

2.Gansu Engn Lab Potato Germplasm Resources Innovat, Lanzhou 730070, Peoples R China

3.Natl Potato Germplasm Resources Observat & Expt St, Weiyuan 748200, Peoples R China

关键词： Potato; Anthocyanins; miRNA; Target genes; Comparative transcriptomics

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN： 0137-5881

年卷期： 2023 年 45 卷 5 期

页码：

收录情况： SCI

摘要： Colored potatoes are rich in anthocyanins and polyphenols, which have high nutritional and health values. miRNAs are closely linked to growth and development, metabolic regulation and various stress response activities in plants. However, the mechanism by which miRNAs regulate potato color remains unclear. In this study, 12 transcriptomic libraries were constructed using a comparative transcriptomics approach to mine miRNAs and their targets associated with anthocyanin formation in potato by comparing anthocyanin levels in four potato varieties. These analyses yielded 167.3 M clean reads and identified 5358 miRNAs, of which 4199 were newly discovered. Relative to group W (LK99), 1235, 1533 and 921 differentially expressed miRNAs (DEmiRNAs) were obtained by the stringent identification threshold in groups B (Black kingkong), R (Red beauty) and Y (Longshu No.7), respectively, and 236 shared DEmiRNAs were screened. Some important miRNAs and target genes were screened by combined analysis, such as miR858, miR172, miR156, miR157, APS1, ABCB4, SPL, RPP13, etc. Several key regulatory pathways were identified by functional annotation analysis, such as metabolic pathways, secondary metabolic pathways, glutathione metabolism, phenylalanine metabolism and cysteine and methionine metabolism. These results provide a comprehensive insight into the miRNAs that regulate potato color formation and will provide a scientific basis for developing colorful or functional potatoes.

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