中国热带农业科学院机构知识库

Ces44T as an endogenous reference gene in real-time quantitative PCR detection of tiger nut (Cyperus esculentus) ingredients in food

收藏
分享
全文链接

文献类型：外文期刊

作者： Pu, Haozhen ¹ ; Xiao, Yanhua ¹ ; Xie, Qingqing ¹ ; Zou, Zhi ² ; Wang, Xiaohui ¹ ; Liang, Qianqian ¹ ; Zhao, Yongguo ³ ; Cheng, Guojun ¹ ; Zhang, Li ¹ ;

作者机构： 1.South Cent Minzu Univ, Coll Life Sci, Hubei Prov Key Lab Protect & Applicat Special Plan, Wuhan 430074, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya Res Inst, Haikou 571101, Hainan, Peoples R China

3.Guangdong Univ Petrochem Technol, Maoming 525000, Guangdong, Peoples R China

关键词： Tiger nut; Endogenous reference gene; Food adulteration; TaqMan real-time quantitative PCR (TaqMan; qPCR); Ces44T gene

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （影响因子：4.6；五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2024 年 134 卷

页码：

收录情况： SCI

摘要： Tiger nut (Cyperus esculentus) is a highly adaptable and nutritious plant that can be used to produce healthenhancing food products, edible oil, and functional ingredients. To ensure transparency, reliability, and accurate labeling throughout the food supply chain, accurate methods for identifying food ingredients are indispensable. In this study, the Ces44T gene was identified and validated as the endogenous reference gene for tiger nut. A real-time quantitative PCR assay targeting the Ces44T gene was developed, demonstrating exceptional specificity in distinguishing tiger nut from other plant species, and exhibiting remarkable stability across different tiger nut cultivars. Standard curves were established to validate the reliability and repeatability of the assay, displaying good linearity (R-2 > 0.99) and PCR efficiency (90-110 %). The assay was estimated to have a limit of detection (LOD) of 0.0033 ng of tiger nut genomic DNA per reaction and a limit of quantification (LOQ) of 0.013 ng. We applied this assay to both laboratory-prepared samples and commercial food products, confirming its excellent specificity and robust performance. In conclusion, the developed real-time quantitative PCR assay provides an accurate means for the identification and quantification of tiger nut ingredients in various samples.

相关文献

作者其他论文更多>>

Insights into membrane-bound fatty acid desaturase genes in tigernut (Cyperus esculentus L.), an oil-rich tuber plant in Cyperaceae

作者：Zou, Zhi;Fu, Xiaowen;Li, Chunqiang;Huang, Jiaquan;Zhao, Yongguo;Huang, Jiaquan;Huang, Jiaquan;Zhao, Yongguo

关键词：Cyperus esculentus; Vegetative tissue; Underground tuber; FAD; Phylogenomics; Orthologous analysis; Syntenic analysis
LC/MS and MALDI-MSI reveal the different distribution of terpenoids in Glycine max and Glycine soja seeds

作者：Yu, Qi;Ren, Zhentao;Liu, Laipan;Zhang, Li;Yin, Xin;Shen, Wenjing;Fang, Zhixiang;Liu, Biao;Jia, Ruizong;Liu, Biao

关键词：Soybeans; Terpenoids; Metabolomics; Flavor; Stress resistance
Prediction of quality traits in packaged mango by NIR spectroscopy

作者：Ding, Fangchen;Chen, Xiao;Lan, Weijie;Pan, Leiqing;Ding, Fangchen;Chen, Xiao;Tu, Kang;Lan, Weijie;Pan, Leiqing;Ding, Fangchen;Garcia-Martin, Juan Francisco;Zhang, Li;Xu, Zhi;Lv, Daizhu

关键词：Mango quality; Paper bag packaging; NIR spectroscopy; Non-invasive analysis; Deep learning; Spectral filtering
Phenolic profiles in the peels of three wampee (Clausena lansium (Lour.) Skeels) fruit cultivars and immune-modulatory activity through the cluster of differentiation 14-mitogen activated protein kinase/nuclear factor kappa B pathways

作者：Zeng, Fanke;Li, Ruyi;Chen, Mianhong;Liang, Yuwei;Zhang, Li;Li, Yingying;Peng, Shaodan;Li, Jihua;Zhou, Wei;Zhu, Yuxiang

关键词：Wampee peels; Phenolic compounds; Immune-modulatory activity; RAW 264.7 macrophages; CD14 receptor; MAPK/NF-kappa B signaling pathways
Insights into the Stearoyl-Acyl Carrier Protein Desaturase (SAD) Family in Tigernut (Cyperus esculentus L.), an Oil-Bearing Tuber Plant

作者：Zou, Zhi;Fu, Xiaowen;Li, Chunqiang;Yi, Xiaoping;Huang, Jiaquan;Zhao, Yongguo;Huang, Jiaquan;Zhao, Yongguo

关键词：vegetative tissue; underground tuber; oleic acid; FAB2; AAD; structural variation; expression divergence
Integrative Analysis Provides Insights into Genes Encoding LEA_5 Domain-Containing Proteins in Tigernut (Cyperus esculentus L.)

作者：Zou, Zhi;Fu, Xiaowen;Yi, Xiaoping;Li, Chunqiang;Huang, Jiaquan;Zhao, Yongguo;Huang, Jiaquan;Huang, Jiaquan;Zhao, Yongguo

关键词：Cyperaceae; vegetative tissue; underground tuber; desiccation tolerance; late embryogenesis-abundant protein; phylogenomics
The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus

作者：Zou, Zhi;Fu, Xiaowen;Li, Na;Zheng, Panyan;Wang, Ruiling;Li, Na;Zheng, Panyan;Wang, Ruiling;Liu, Hongyan;Huang, Jiaquan;Zhao, Yongguo

关键词：Oil crop; Oil accumulation; Lipid droplet; Encapsulation; Oleosin; WRINKLED3; Regulatory mechanism

Ces44T as an endogenous reference gene in real-time quantitative PCR detection of tiger nut (Cyperus esculentus) ingredients in food

作者其他论文 更多>>

Insights into membrane-bound fatty acid desaturase genes in tigernut (Cyperus esculentus L.), an oil-rich tuber plant in Cyperaceae

LC/MS and MALDI-MSI reveal the different distribution of terpenoids in Glycine max and Glycine soja seeds

Prediction of quality traits in packaged mango by NIR spectroscopy

Phenolic profiles in the peels of three wampee (Clausena lansium (Lour.) Skeels) fruit cultivars and immune-modulatory activity through the cluster of differentiation 14-mitogen activated protein kinase/nuclear factor kappa B pathways

Insights into the Stearoyl-Acyl Carrier Protein Desaturase (SAD) Family in Tigernut (Cyperus esculentus L.), an Oil-Bearing Tuber Plant

Integrative Analysis Provides Insights into Genes Encoding LEA_5 Domain-Containing Proteins in Tigernut (Cyperus esculentus L.)

The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus

意 见 箱

作者其他论文更多>>

意见箱