Characterization of the Glutamate Decarboxylase (GAD) Gene and Functional Analysis of DlGAD3 in the Accumulation of γ-Aminobutyric Acid in Longan (Dimocarpus longan Lour.) Pulp
文献类型： 外文期刊
作者： Wei, Weilin 1 ; Zhang, Tingting 1 ; Chen, Yongping 1 ; Zhou, Ziqi 1 ; Su, Wenbing 1 ; Xu, Qizhi 1 ; Zhang, Yaling 1 ; Zheng, Shaoquan 1 ; Jiang, Jimou 1 ; Deng, Chaojun 1 ;
作者机构： 1.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou 350013, Peoples R China
关键词：
longan; gamma-aminobutyric acid (GABA); glutamate decarboxylase (
期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 11 卷 6 期
页码：
收录情况： SCI
摘要： gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid, with many regulatory effects in humans. It aids in regulating blood glucose levels and pressure and is widely recognized for its ability to promote cognitive balance through the alleviation of stress and improvements in sleep quality. The GABA content of longan pulp is higher than that of many other fruits and vegetables; however, much is still unknown about GABA's biosynthesis in longan. In this study, we found that the GABA content of 'Baoshi No. 1' (BS1) pulp was significantly higher than that of 'Chunxiang' (CX) pulp. The GAD activity was higher in BS1 pulp than CX pulp, while there was no significant difference in the GABA-T activity. Additionally, five GAD genes were identified in longan, and an analysis of their transcriptional levels showed that only the expression level of DlGAD3 corresponded to the GABA content and GAD activity. DlGAD3 was localized in the cytoplasm, and its transient overexpression promoted an increase in the GABA content in Nicotiana benthamiana leaves. Overall, our results show that DlGAD3 is able to promote the accumulation of GABA and may play a major role in its biosynthesis in longan pulp.
- 相关文献
作者其他论文 更多>>
-
Metabolomic and transcriptomic analyses provide insights into the red pigmentation in loquat (Eriobotrya japonica) peel
作者：Zhang, Yaling;Chen, Xiuping;Su, Wenbing;Deng, Chaojun;Jiang, Jimou;Zheng, Shaoquan
关键词：loquat; RNA-seq; anthocyanin biosynthesis; EjMYB10; anthocyanin-targeted metabolome
-
MaERF9 and MaERF113 transcription factors involve in chilling injury development by regulating membrane lipid metabolism of postharvest banana fruit
作者：Bai, Lijuan;Wu, Yanting;Lin, Hetong;Fan, Zhongqi;Bai, Lijuan;Wu, Yanting;Lin, Hetong;Fan, Zhongqi;Su, Wenbing
关键词：Banana fruit; Chilling injury; Membrane lipid metabolism; ERF transcription factors; Transcriptional regulation
-
Occurrence of White Flesh Color and Refreshing Flavor Following Phytoene Synthase 2A Gene Variation in Loquat Fruit
作者：Su, Wenbing;Chen, Xiuping;Wei, Weilin;Kou, Yidan;Deng, Chaojun;Lin, Han;Chen, Yongping;Xu, Qizhi;Tong, Zhihong;Jiang, Jimou;Wu, Lulu;Zhu, Changqing;Xu, Changjie
关键词：loquat; white-fleshed cultivar;
PSY2A ; refreshing flavor; metabolome -
Genome-wide identification of CONSTANS-like genes in Dimocarpus longan and functional characterization of DlCOL9 revealing its role in floral induction
作者：He, Dayi;Wei, Chunyan;Liang, Fan;Huang, Yuanyuan;Fang, Ting;Zeng, Lihui;He, Dayi;Wei, Chunyan;Liang, Fan;Huang, Yuanyuan;Fang, Ting;Zeng, Lihui;Deng, Chaojun
关键词：Dimocarpus longan; CONSTANS-LIKE gene; Floral induction; Photoperiod
-
Methyl jasmonate
(MeJA) treatment functions as a promising method to remain postharvest loquat fruit quality via regulating sucrose metabolism作者：Tong, Zhihong;Wan, Jiahui;Lin, Han;Zhang, Rui;Lin, Hetong;Fan, Zhongqi;Tong, Zhihong;Wan, Jiahui;Lin, Han;Zhang, Rui;Lin, Hetong;Fan, Zhongqi;Tong, Zhihong;Lin, Han;Zhang, Rui;Jiang, Jimou;Su, Wenbing
关键词：Loquat fruit; storage; fruit quality; methyl jasmonate (MeJA); sucrose metabolism
-
Construction of high-density bin genetic map and QTL mapping of fruit aroma in longan (Dimocarpus longan Lour.)
作者：Hu, Wenshun;Deng, Chaojun;Xu, Qizhi;Jiang, Jimou;Chen, Xiuping;Zheng, Shaoquan;Zhang, Qing;Zhang, Jisen;Li, Jianguo
关键词：longan; high-density bin map; characteristic aroma components; QTL mapping; candidate gene
-
Two FT genes synergistically regulate the reproductive transition of loquat
作者：Jiang, Yuanyuan;Peng, Ze;Yuan, Yuan;Ma, Chongjian;Jiang, Yuanyuan;Jiang, Yuanyuan;Zhu, Yunmei;Peng, Ze;Su, Wenbing;Peng, Jiangrong;Zhang, Ling;Zhang, Zhike;Yang, Xianghui;Gao, Yongshun;Lin, Shunquan;Jiang, Yuanyuan;Ma, Chongjian;Zhu, Yunmei;Su, Wenbing;Gao, Yongshun
关键词：Loquat; Flowering time; GA3; Short day; FT; AP1
