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CRISPR/Cas9 Technology and Its Utility for Crop Improvement

文献类型： 外文期刊

作者： Liu, Hua 1 ; Chen, Wendan 2 ; Li, Yushu 3 ; Sun, Lei 1 ; Chai, Yuhong 1 ; Chen, Haixia 1 ; Nie, Haochen 1 ; Huang, Conglin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

2.Beijing Forestry Univ, Dept Food Sci & Engn, Coll Biol Sci & Biotechnol, Beijing Key Lab Forest Food Proc & Safety, Beijing 100083, Peoples R China

3.Beijing Vocat Coll Agr, Beijing 100097, Peoples R China

关键词： CRISPR; Cas9; gene editing; crop improvement; plant breeding; gene function

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 18 期

页码：

收录情况： SCI

摘要： The rapid growth of the global population has resulted in a considerable increase in the demand for food crops. However, traditional crop breeding methods will not be able to satisfy the worldwide demand for food in the future. New gene-editing technologies, the most widely used of which is CRISPR/Cas9, may enable the rapid improvement of crop traits. Specifically, CRISPR/Cas9 genome-editing technology involves the use of a guide RNA and a Cas9 protein that can cleave the genome at specific loci. Due to its simplicity and efficiency, the CRISPR/Cas9 system has rapidly become the most widely used tool for editing animal and plant genomes. It is ideal for modifying the traits of many plants, including food crops, and for creating new germplasm materials. In this review, the development of the CRISPR/Cas9 system, the underlying mechanism, and examples of its use for editing genes in important crops are discussed. Furthermore, certain limitations of the CRISPR/Cas9 system and potential solutions are described. This article will provide researchers with important information regarding the use of CRISPR/Cas9 gene-editing technology for crop improvement, plant breeding, and gene functional analyses.

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