Ultrasensitive determination of alpha-glucosidase activity using CoOOH nanozymes and its application to inhibitor screening
文献类型： 外文期刊
作者： Ma, Demiao 1 ; Ge, Jia 1 ; Wang, Ang 1 ; Li, Jingxian 2 ; Yang, Hongfen 5 ; Zhai, Wenlei 4 ; Cai, Ren 2 ;
作者机构： 1.Zhengzhou Univ, Coll Chem, Zhengzhou 450001, Peoples R China
2.Hunan Univ, Coll Chem & Chem Engn, Coll Biol Coll Mat Sci & Engn, Mol Sci & Biomed Lab,State Key Lab Chemo Biosensin, Changsha 410082, Peoples R China
3.Hunan Univ, Collaborat Res Ctr Mol Engn Theranost, Changsha 410082, Peoples R China
4.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China
5.Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China
期刊名称：JOURNAL OF MATERIALS CHEMISTRY B （ 2022影响因子：7.0； 五年影响因子：6.5 ）
ISSN： 2050-750X
年卷期： 2023 年 11 卷 12 期
收录情况： SCI
摘要： In this work, a novel method for the colorimetric sensing of alpha-glucosidase (alpha-Glu) activity was developed based on CoOOH nanoflakes (NFs), which exhibit efficient oxidase-mimicking activity. Colorless 3,3 ',5,5 '-tetramethylbenzidine (TMB) can be oxidized by CoOOH NFs into blue-colored oxidized TMB (oxTMB) in the absence of H2O2. l-Ascorbic acid-2-O-alpha-d-glucopyranose (AAG) can be hydrolysed by alpha-glucosidase to produce ascorbic acid, resulting in a significant decrease of catalytic activity of CoOOH NFs. Thus, a colorimetric alpha-glucosidase activity detection method was designed with a limit of detection of 0.0048 U mL(-1). Furthermore, the designed sensing platform exhibits favorable applicability for the alpha-glucosidase (alpha-Glu) activity assay in real samples. Meanwhile, this method can be expanded to study the inhibitors of alpha-Glu. Finally, the as-proposed method combined with a smartphone would be a color recognizer, which was successfully applied for the determination of alpha-Glu activity in human serum samples.
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