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The Exocyst Complex Subunit EXO70E1-V From Haynaldia villosa Interacts With Wheat Powdery Mildew Resistance Gene CMPG1-V

文献类型： 外文期刊

作者： Zhao, Jia 1 ; Zhang, Heng 1 ; Zhang, Xu 1 ; Wang, Zongkuan 1 ; Niu, Ying 1 ; Chen, Yiming 1 ; Sun, Li 1 ; Wang, Haiyan 1 ; W 1 ;

作者机构： 1.Nanjing Agr Univ, JiangSu Collaborat Innovat Ctr Modern Crop Prod, Cytogenet Inst, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China

2.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangdong Lab Lingnan Modern Agr, Lab Seed Sci & Technol,Guangdong Key Lab Plant Mo, Guangzhou, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

关键词： CMPG1-V; Haynaldia villosa; interaction protein; powdery mildew

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： EXO70 belongs to the exocyst complex subunit that plays a critical role in regulating plant cell polarity establishment and defense response. A previous study proved that the E3 ligase CMPG1-V from Haynaldia villosa, a diploid wheat relative, positively regulates the resistance to wheat powdery mildew (Pm), caused by fungus Blumeria graminis f.sp tritici (Bgt). In this study, a member of EXO70 superfamily named EXO70E1-V was isolated from H. villosa, and EXO70E1-V interacted with CMPG1-V were shown by yeast two-hybrid (Y2H), pull-down assay, bimolecular fluorescence complementation (BiFC) assay, and luciferase complementation imaging (LCI) assay. It is localized in various subcellular organs, i.e., plasma membrane (PM) and endoplasmic reticulum. Co-expression of EXO70E1-V and CMPG1-V showed dot-like structure fluorescence signals that were mainly in PM and nucleus. Expression of EXO70E1-V was relatively higher in leaf and was significantly induced by Bgt infection and exogenous application of hormones such as salicylic acid. Transient or stable overexpression of EXO70E1-V could not enhance/decrease the Pm resistance level, suggesting overexpression of EXO70E1-V alone has no impact on Pm resistance in wheat.

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