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Expression analysis and functional characterization of tomato Tubby-like protein family

文献类型： 外文期刊

作者： Zhang, Jiaojiao 1 ; Wang, Xinman 1 ; Dong, Xiaonan 1 ; Wang, Fei 1 ; Cao, Lingling 3 ; Li, Shuangtao 4 ; Liu, Ziji 5 ; Zhang, Xichun 6 ; Guo, Yang-Dong 1 ; Zhao, Bing 1 ; Zhang, Na 1 ;

作者机构： 1.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China

2.China Agr Univ, Sanya Inst, Sanya 572000, Peoples R China

3.Beijing Agr Technol Extens Stn, Beijing 100029, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

5.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China

6.Beijing Agr Univ, Sch Plant Sci & Technol, Beijing 102206, Peoples R China

关键词： E3 ubiquitin ligase; F -box protein; Subcellular localization; Solanum lycopersicum; Transactivation activity; Tubby domain

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 324 卷

页码：

收录情况： SCI

摘要： Tubby-like protein (TLP) plays an important role in plant growth and development. In this investigation, the characteristics of 11 members in the SlTLP family were studied. SlTLP genes were classified into two subgroups, and the members containing the F-box domain were renamed SlTLFPs. Subcellular localization indicated that most of the SlTLPs were localized in the nucleus. Expression pattern analysis revealed that eight genes (SlTLFP1, 3, 5, 7-10, and SlTLP11) showed differential expression across various tissues, while SlTLFP2, 4, and 6 were widely expressed in all the organs tested. Most SlTLP genes were induced by biotic and abiotic stress treatments such as Botrytis cinerea, temperature, MeJA, and ABA. TLP proteins in tomato have no transcriptional activation activity, and most members with an F-box domain could interact with SUPPRESSOR OF KINETOCHORE PROTEIN 1 (SlSkp1) or Cullin1 (Cul1) or both. Experiments on CRISPR edited SlTLFP8 showed that the N-terminal Fbox domain was necessary for its function such as DNA ploidy and stomata size regulation. Our findings suggested that the F-box domain interacts with Skp1 and Cul1 to form the SCF complex, suggesting that SlTLFPs, at least SlTLFP8, function mainly through the F-box domain as an E3 ligase.

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