Progesterone and Androstenedione Are Important Follicular Fluid Factors Regulating Porcine Oocyte Maturation Quality
文献类型： 外文期刊
作者： Zhao, Huaxing 1 ; He, Xiaohua 1 ; Zhang, Xianjun 1 ; Shi, Junsong 4 ; Zhou, Rong 5 ; Mai, Ranbiao 5 ; Su, Qiaoyun 5 ; Cai, Gengyuan 1 ; Huang, Sixiu 1 ; Xu, Zheng 1 ; Wu, Zhenfang 1 ; Li, Zicong 1 ;
作者机构： 1.South China Agr Univ, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Peoples R China
2.South China Agr Univ, State Key Lab Livestock & Poultry Breeding, Guangzhou 510642, Peoples R China
3.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou 510642, Peoples R China
4.Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Peoples R China
5.Guangdong Wens Breeding Swine Technol Co Ltd, Yunfu 527439, Peoples R China
6.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China
关键词： pig; oocyte maturation; follicular fluid; maturation medium; metabolomics
期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2023 年 13 卷 11 期
页码：
收录情况： SCI
摘要： Simple SummaryThe quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in FF during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate or subsequent embryo developmental competence.Oocytes matured in vitro are useful for assisted human and farm animal reproduction. However, the quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, untargeted metabolomics was used to detect the changes in the metabolites in the follicular fluid (FF) during in vivo pig oocyte maturation and in the culture medium during in vitro maturation. Our results showed that the total metabolite changing profile of the in vivo FF was different from that of the in vitro maturation medium, but the levels of 23 differentially expressed metabolites (DEMs) changed by following the same trend during both in vivo and in vitro pig oocyte maturation. These 23 metabolites may be important regulators of porcine oocyte maturation. We found that progesterone and androstenedione, two factors in the ovarian steroidogenesis pathway enriched from the DEMs, were upregulated in the FF during in vivo pig oocyte maturation. The levels of these two factors were 31 and 20 fold, respectively, and they were higher in the FF than in the culture medium at the oocyte mature stage. The supplementation of progesterone and androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence. Our finding suggests that a metabolic abnormality during in vitro pig oocyte maturation affects the quality of the matured oocytes. This study identified some important metabolites that regulate oocyte maturation and their developmental potential, which will be helpful to improve assisted animal and human reproduction.
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