Sensitive DNA Detection using a Branched DNA as a Sensor Coupled with Hybridization Chain Reaction
文献类型： 外文期刊
作者： Zhang, Zhikun 1 ; Zhang, Liu 1 ; Liu, Yumin 1 ; Hu, Cuixia 1 ; Liu, Qingju 2 ;
作者机构： 1.Hebei Univ Sci & Technol, Sch Chem & Pharmaceut Engn, Shijiazhuang 050018, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
关键词： amplification; branched DNA; disease's diagnosis; nucleic acids; sensor
期刊名称：CHEMISTRYSELECT （ 影响因子：2.307； 五年影响因子：2.112 ）
ISSN： 2365-6549
年卷期： 2022 年 7 卷 44 期
页码：
收录情况： SCI
摘要： Nucleic acids detection is becoming increasingly vital for disease's diagnosis. A sensitive, convenient and simple detection method provide the possibility of self-diagnosis to prevent the disease's development. Herein, we designed a novel branched DNA nanostructure as probe, which could induce DNA hybridization chain reaction in the presence of target DNA. The strategy as amplifier enhanced the electrochemical signal of target DNA for sensitive nucleic acids detection. In this system, the branched DNA probe possessed more capturing points for Target DNA than previous single-stranded DNA. Target DNA as trigger were used to induce hybridization chain reaction in three dimensions. The limit of detection of this strategy can reach to 1 pM with excellent specificity and selectivity. Meanwhile, the linear working range was from 10 pM to 100 mu M. The biosensor had a great promising in the determination of nucleic acids for disease's diagnosis.
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