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Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck

文献类型： 外文期刊

作者： Du, Xue 1 ; Lai, Shujing 3 ; Zhao, Wanqiu 4 ; Xu, Xiaoqin 5 ; Xu, Wenwu 1 ; Zeng, Tao 1 ; Tian, Yong 1 ; Lu, Lizhi 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang A&F Univ, Coll Anim Sci & Technol, Coll Vet Med, Hangzhou, Peoples R China

3.Shanghai Jiao Tong Univ, Shanghai Inst Immunol, Sch Med, Shanghai, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310022, Zhejiang, Peoples R China

5.China West Normal Univ, Inst Ecol, Nanchong 637002, Sichuan, Peoples R China

关键词： Ceased-laying duck; Single-cell RNA-seq; Cell heterogeneity; Hepatocytes; Cell cluster

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background: In the late phase of production, ducks untimely cease laying, leading to a lower feed conversion. Liver plays a vital role in the synthesis and transport of yolk materials during egg formation in birds. However, the molecular mechanism of liver in ceased-laying duck is far from clear, higher resolution and deeper analysis is needed. Sing-cell RNA-sequencing of 10 x Genomics platform can help to map the liver single cell gene expression atlas of Shaoxing duck and provide new insights into the liver between egg-laying and ceased-laying ducks. Results: About 20,000 single cells were profiled and 22 clusters were identified. All the clusters were identified as 6 cell types. The dominant cell type is hepatocyte, accounted for about 60% of all the cells. Of note, the heterogeneity of cells between egg-laying duck and ceased-laying duck mainly occurred in hepatocytes. Cells of cluster 3 and 12 were the unique hepatocyte states of egg-laying ducks, while cells of cluster 0 and 15 were the unique hepatocyte states of ceased-laying ducks. The expression mode of yolk precursor transporters, lipid metabolizing enzymes and fibrinogens were different in hepatocytes between egg-laying duck and ceased-laying duck. APOV1, VTG2, VTG1, APOB, RBP, VTDB and SCD might be activated in egg-laying ducks, while APOA1, APOA4, APOC3, FGB and FGG might be activated in ceased-laying ducks. Conclusions: Our study further proofs that APOV1 and APOB play key roles in egg production, rather than APOA1 and APOA4. It is also the first to detect a correlation between the higher expression of APOC3, FGB, FGG and ceased-laying in duck.

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