The long non-coding RNA MSTRG.32189-PcmiR399b-PcUBC24 module regulates phosphate accumulation and disease resistance to Botryosphaeria dothidea in pear
文献类型： 外文期刊
作者： Yang, Yuekun 1 ; Lv, Shamei 1 ; Huang, Xiaosan 4 ; He, Ying 1 ; Zhang, Xiaoyan 1 ; Liu, Yu 1 ; Wang, Guoping 1 ; Hong, Ni 1 ; Wang, Liping 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China
3.Hebei Acad Agr & Forestry Sci, Shijiazhuang Fruit Tree Res Inst, Shijiazhuang 050061, Peoples R China
4.Nanjing Agr Univ, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）
ISSN： 2662-6810
年卷期： 2025 年 12 卷 4 期
页码：
收录情况： SCI
摘要： Pear ring rot disease (Botryosphaeria dothidea) is a significant threat to the healthy development of the pear industry. Recent research has identified the functional role of long non-coding RNAs (lncRNAs) in various biological processes of plants. The role of lncRNAs in the pear defense response remains unknown. In this study, transcriptome sequencing was used to analyze lncRNAs in pear stem infected with B. dothidea. It identified 3555 lncRNAs, of which 286 were significantly differentially expressed. GO and KEGG analyses showed that cis- and trans-regulated target genes were enriched in multiple disease resistance-related pathways. More specifically, MSTRG.32189, predicted as an endogenous target mimic (eTM), was significantly down-regulated in response to B. dothidea infection, and was confirmed to inhibit the cleavage effect of PcmiR399b on PcUBC24. OE-MSTRG.32189 transgenic Arabidopsis exhibited lower Pi content and weaker disease resistance to Botrytis cinerea compared with wild type. In pear callus, overexpression of MSTRG.32189 negatively regulated PcmiR399b, which decreased Pi content and reduced disease resistance. Overexpressing PcmiR399b in pear callus exhibited the opposite effects compared with OE-MSTRG.32189. Overexpression and knockout of PcUBC24 further clarified that PcUBC24 negatively regulates Pi content and disease resistance to B. dothidea infection. Furthermore, the ROS levels and expressions of disease resistance pathway-related genes were regulated by the MSTRG.32189-PcmiR399b-PcUBC24 module in transgenic pear callus, which contributed to disease resistance. Overall, our results demonstrated the role of lncRNAs in the pear defense response, revealing that the MSTRG.32189-PcmiR399b-PcUBC24 module regulates phosphate accumulation and disease resistance to B. dothidea infection in pear.
- 相关文献
作者其他论文 更多>>
-
PcMYB44 regulated host resistance to Botryosphaeria dothidea through activation of lignin biosynthesis and disease-resistance gene expression in pear
作者：Lv, Shamei;Yang, Yuekun;Zhang, Xiaoyan;He, Ying;Wang, Guoping;Hong, Ni;Wang, Liping;Lv, Shamei;Yang, Yuekun;Zhang, Xiaoyan;He, Ying;Wang, Guoping;Hong, Ni;Wang, Liping;Yang, Yuekun
关键词：Pear; Botryosphaeria dothidea; PcMYB44; Laccase; Lignification; Disease resistance
-
Rapid evaluation of drought tolerance of winter wheat cultivars under water-deficit conditions using multi-criteria comprehensive evaluation based on UAV multispectral and thermal images and automatic noise removal
作者：Wu, Yongfeng;Ma, Juncheng;Zhang, Wenying;Liu, Binhui;Wang, Bianyin;Chen, Zhaoyang;Sun, Liang;Liu, Yu;Ma, Juncheng;Liu, Binhui
关键词：UAV multispectral and thermal images; Automatic image segmentation; Multi -criteria comprehensive evaluation; Drought tolerance
-
Estimation of Winter Wheat Yield Using Multiple Temporal Vegetation Indices Derived from UAV-Based Multispectral and Hyperspectral Imagery
作者：Liu, Yu;Sun, Liang;Liu, Binhui;Zhang, Wenying;Wang, Bianyin;Chen, Zhaoyang;Liu, Binhui;Zhang, Wenying;Wang, Bianyin;Chen, Zhaoyang;Wu, Yongfeng;Ma, Juncheng
关键词：UAV; multiple temporal; hyperspectral imagery; field plot scale; vegetation index; yield estimation
-
Heterogeneity of soil bacterial and bacteriophage communities in three rice agroecosystems and potential impacts of bacteriophage on nutrient cycling
作者：Wang, Yajiao;Liu, Yu;Wu, Nan;Liu, Wenwen;Wang, Xifeng;Wang, Yajiao;Wu, Yuxing
关键词：Paddy soil; Bacteriophage; Bacteriophage-bacteria interaction; Auxiliary metabolic genes; Nitrogen cycling
-
Economic Assessment of Food Legumes Breeding in China: Evidence Using a Provincial Level Dataset
作者：Ma, Jiliang;Zhang, Huijie;Khan, Nawab;Tian, Jing;Wang, Lixia;Wu, Jing;Cheng, Xuzhen;Ren, Guixing;Chen, Xin;Liu, Yujiao;He, Yuhua;Liu, Changyan;Xia, Xianfei;Guo, Yanping;Zhang, Xiaoyan;Zhou, Bin;Liu, Zhenxing;Lian, Rongfang
关键词：food legumes; breeding; research and development; China; global Moran's I index
-
February orchid cover crop improves sustainability of cotton production systems in the Yellow River basin
作者：Wang, Zhanbiao;Xiong, Shiwu;Li, Xiaofei;Han, Yingchun;Wang, Guoping;Feng, Lu;Fan, Zhengyi;Lei, Yaping;Yang, Beifang;Xing, Fangfang;Xin, Minghua;Du, Wenli;Li, Yabing;Zhai, Lichao
关键词：Carbon footprint; Nitrogen footprint; Environmental damage costs; Net ecosystem economic benefit
-
Seed biostimulant Bacillus sp. MGW9 improves the salt tolerance of maize during seed germination
作者：Li, Heqin;Liu, Yu;Zhang, Haiyan;Jiang, Xuwen;Yue, Haiwang;Li, Li;Wang, Jianhua;Jiang, Xuwen
关键词：Salt stress; Seed biostimulant; Seed germination; Zea mays; Bacillus sp
