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Proactive Resistance Management Studies Highlight the Role of Cytochrome P450 Genes in the Resistance of Tuta absoluta Against Tetraniliprole

文献类型： 外文期刊

作者： Ullah, Farman 1 ; Ullah, Zeeshan 3 ; Gul, Hina 1 ; Li, Xiaowei 1 ; Pan, Yuhan 1 ; Zhang, Haixia 1 ; Zhang, Zhijun 1 ; Huang, Jun 1 ; Emmanouil, Roditakis 4 ; Guedes, Raul Narciso C. 5 ; Desneux, Nicolas 6 ; Lu, Yaobin 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Qual & Safety Agroprod, Key Lab Biotechnol Plant Protect MOA China, Hangzhou 310021, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China

3.Abdul Wali Khan Univ Mardan, Dept Entomol, Mardan 23200, Khyber Pakhtunk, Pakistan

4.Hellenic Mediterranean Univ, Sch Agr Sci, Dept Agr, Iraklion 71410, Greece

5.Univ Fed Vicosa, Dept Entomol, BR-36570900 Vicosa, MG, Brazil

6.Univ Cote Azur, INRAE, CNRS, UMR ISA, F-06000 Nice, France

关键词： nanomaterial; RNA interference; insecticide resistance; cytochrome P450; diamide

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 11 期

页码：

收录情况： SCI

摘要： The diamide insecticide tetraniliprole is a valuable tool for managing major insect pests like the invasive tomato pinworm, Tuta absoluta (Meyrick). However, the mechanisms underlying tetraniliprole resistance, as well as its associated fitness costs, remain unclear. In this study, we assessed the fitness of tetraniliprole-resistant (TetraRS) and susceptible (SS) strains of T. absoluta and conducted Illumina RNA-seq to compare their transcriptomes. We also used nanocarrier-mediated RNA interference (RNAi) to knockdown P450 genes and evaluate their role in tetraniliprole resistance. After eight generations of selection, T. absoluta developed a 20.80-fold resistance to tetraniliprole, accompanied by fitness costs. RNA-seq analysis revealed 3332 differentially expressed genes (DEGs), with 1707 upregulated and 1625 downregulated in the TetraRS compared to the SS strain. Gene Ontology (GO) annotations showed significant enrichment in categories related to metabolic processes, cellular processes, catalytic activity, cellular anatomical entity, and binding. These genes were also identified in key KEGG pathways such as cytochrome P450, drug metabolism, carbon metabolism, oxidative phosphorylation, fatty acid metabolism, and protein processing. RT-qPCR analysis confirmed that P450 genes (CYP405D1, CYP6AB269, and CYP4AU1) were upregulated in TetraRS insects, in line with the RNA-seq results. Cytochrome P450 activity was significantly higher in the TetraRS strain than in the SS strain. Notably, nano-encapsulated dsRNA targeting these overexpressed P450 genes increased the susceptibility of T. absoluta to tetraniliprole. Further, cytochrome P450 activity was significantly reduced following silencing of P450 genes. These findings suggest that multiple genes and pathways, particularly P450 genes, contribute to tetraniliprole resistance in T. absoluta. This study provides valuable insights into the molecular mechanisms underlying insecticide resistance in this key pest species.

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