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Rapid detection of pigeon Megrivirus using TaqMan real-time PCR technology

文献类型： 外文期刊

作者： Zhang, Jinpeng 1 ; Fu, Huanru 2 ; Chen, Cuiteng 1 ; Jiang, Jinxiu 1 ; Lin, Yusheng 1 ; Jiang, Bin 1 ; Lin, Lin 1 ; Hu, Qilin 1 ; Wan, Chunhe 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Peoples R China

2.Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China

3.Fujian Anim Dis Control Technol Dev Ctr, Fujian Key Lab Avian Dis Control & Prevent, Fujian Key Lab Anim Genet & Breeding, Fuzhou 350013, Peoples R China

关键词： racing pigeons; Megrivirus; TaqMan-qPCR; vertical transmission

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 11 期

页码：

收录情况： SCI

摘要： Megriviruses have been identified from fecal samples in wild pigeons in Hong Kong (China) and Hungary. In this study, the genomic sequences of pigeon Megriviruses (PiMeVs) were downloaded from GenBank and compared. Based on the genetic comparison results, a pair of primers and TaqMan probe were designed based on the conserved sequences of the 3C gene (located in the P3 gene coding region), and a TaqMan real-time PCR method (TaqMan-qPCR) was established. The standard curve of the TaqMan-qPCR had an axial intercept of 39.74 and a slope of-3.2475 with a linear correlation (R2) of 1.00 and an efficiency of 103.2%. No cross-amplification signal was found from other pigeon viruses (such as avian influenza virus, pigeon paramyxovirus type I, pigeon torque teno virus, pigeon adenovirus, and pigeon circovirus). The limit of detection concentration was 53.6 copies/ mL. The intra-and interassay results were less than 1.0% based on the reproducibility test. Furthermore, field samples investigation by the established TaqMan-qPCR method showed that positive signals can be found from racing pigeon fecal samples and embryos. Thus, our data suggested that this visible TaqMan-qPCR method is sensitive, specific, and reproducible. Moreover, we first confirmed the presence of pigeon Megrivirus infection in racing pigeon embryos, indicating that the virus may be vertically transmitted. This study provides a reference basis for further understanding the epidemiology of PiMeVs.

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