H-1 NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence
文献类型： 外文期刊
作者： Liu, Juan 1 ; Zhou, Xinqiao 1 ; Chen, Dagang 1 ; Guo, Jie 1 ; Chen, Ke 1 ; Ye, Chanjuan 1 ; Liu, Chuanguang 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Coconstruct Minist & Prov, Minist Agr & Rural Affairs, Rice Res Inst,Guangdong Rice Engn Lab,Guangdong Ke, Guangzhou 510640, Peoples R China
关键词： pomelo; metabolic profiling; NMR; naringin; citric acid; flavor
期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）
ISSN：
年卷期： 2023 年 12 卷 10 期
页码：
收录情况： SCI
摘要： This study investigated metabolite changes in three pomelo cultivars during postharvest senescence using H-1 NMR-based metabolic profiling. Three pomelo cultivars, `Hongroumiyou', `Bairoumiyou' and `Huangroumiyou', abbreviated as "R", "W" and "Y" according to the color of their juice sacs, were stored at 25 degrees C for 90 days, and NMR was applied to determine the metabolite changes in juice sacs during storage. Fifteen metabolites were identified, including organic acids, sugars, amino acids, fatty acids, phenols and naringin. Partial least squares discriminant analysis (PLS-DA) was used to screen the significant metabolites according to the variable importance for the projection (VIP) scores in three pomelo cultivars during 90 days of storage. Additionally, eight metabolites, naringin, alanine, asparagine, choline, citric acid, malic acid, phosphocholine and fi-D-glucose, were screened to be the crucial biomarkers with VIP > 1. The undesirable flavor of "bitter and sour" during the 60 days of storage was mainly attributed to the naringin, citric acid and sugars. According to the correlation analysis, the citric acid content determined by NMR showed a significantly positive relationship with that analyzed by HPLC. These findings suggested that NMR technology was accurate and efficient for metabolomic analysis of pomelo fruit, and the H-1 NMR-based metabolic profiling can be efficient during quality evaluation and useful for improving the fruit flavor quality during postharvest storage.
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