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Essentiality for rice fertility and alternative splicing of OsSUT1

文献类型： 外文期刊

作者： Wang, Xiaowen 1 ; Liu, Xiuli 1 ; Hu, Zhi 1 ; Bao, Shuhui 1 ; Xia, Huihuang 1 ; Feng, Bing 1 ; Ma, Lai 1 ; Zhao, Gengmao 1 ; Zhang, Dechun 4 ; Hu, Yibing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

4.China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Peoples R China

关键词： OsSUT1; Caryopsis; Fertility; Alternative splicing; Rice

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 314 卷

页码：

收录情况： SCI

摘要： Sucrose-proton symporters play important roles in carbohydrate transport during plant growth and development. Their physiological functions have only been partly characterized and their regulation mechanism is largely unclear. Here we report that the knockout of a sucrose transporter gene, OsSUT1, by CRISPR-Cas9 mediated gene editing resulted in a slightly dwarf size and complete infertility of the gene's homozygous mutants. Observation of caryopsis development revealed that the endosperm of OsSUT1 mutants failed to cellularize and did not show any sign of seed-filling. Consistently, OsSUT1 was identified to express strongly in developing caryopsis of wildtype rice, particularly in the nucellar epidermis and aleurone which are critical for the uptake of nutrients into the endosperm. These results indicate that OsSUT1 is indispensable during the rice reproductive stage particularly for caryopsis development. Interestingly, OsSUT1 possesses at least 6 alternative splicing transcripts, including the 4 transcripts deposited previously and the other two identified by us. The differences among these transcripts primarily lie in their coding region of the 3' end and 3' UTR region. Real-time PCR showed that 4 of the 6 transcripts had different expressional patterns during rice vegetative and reproductive growth stages. Given the versatility of the gene, addressing its alternative splicing mechanism may expand our understanding of SUT' s function substantially.

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