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Mapping of resistance genes to powdery mildew based on DNA re-sequencing and bulk segregant analysis in Capsicum

文献类型： 外文期刊

作者： Zhang, Tao 1 ; Bosland, Paul W. 2 ; Ma, Yan 1 ; Wang, Yuhang 1 ; Li, Wei 1 ; Kong, Weifu 1 ; Wei, Min 3 ; Duan, Panpan 3 ; Zhang, Gaoyuan 1 ; Wei, Bingqiang 1 ;

作者机构： 1.Gansu Agr Univ, Coll Hort, Lanzhou 730070, Gansu, Peoples R China

2.New Mexico State Univ, Plant & Environm Sci Dept, POB 30003, Las Cruces, NM 88001 USA

3.Gansu Acad Agr Sci, Vegetable Inst, Lanzhou 730070, Gansu, Peoples R China

关键词： BSA-seq; Molecular markers; Pepper; Powdery mildew; Ubiquitin-conjugating enzyme

期刊名称：PROTOPLASMA （ 影响因子：2.5； 五年影响因子：3.0 ）

ISSN： 0033-183X

年卷期： 2025 年 262 卷 3 期

页码：

收录情况： SCI

摘要： Powdery mildew caused by Leveillula taurica adversely affects the development and growth of pepper plants. However, there have been few reports on the fine mapping and quantitative trait locus (QTLs) gene cloning of resistance genes to powdery mildew in pepper. Herein, an F2 segregating population was constructed using the high resistance material "NuMex Suave Red" and the extremely susceptible material "c89" for bulked segregant analysis and DNA re-sequencing (BSA-seq). Molecular markers were used to achieve fine mapping, followed by expression verification. A major QTL located on chromosome 5 (Chr5, 7.20-11.75 Mb) that is associated with resistance to powdery mildew in pepper was mapped using BSA-seq. A narrow interval of 64.86 kb encompassing five genes was refined using InDel and KSAP molecular markers developed from the QTL region. Among them, the expression of the ubiquitin-conjugating enzyme E2 gene, Capana05g000392, was significantly upregulated in multiple resistant materials. In addition, there was a single nucleotide polymorphism (SNP) of A/G in the 241st position of the CDS sequence of Capana05g000392, which in turn leads to an amino acid polymorphism of M/V between susceptible parent and resistant parent. Overall, these results indicate that the Capana05g000392 gene may serve as a robust potential factor against powdery mildew in pepper. These findings further elucidate the genetic mechanism of resistance to powdery mildew in pepper and facilitate molecular marker-assisted breeding.

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