Ultrasensitive molecular imprinted electrochemical sensor for in vivo determination of glycine betaine in plants
文献类型： 外文期刊
作者： Ai, Geng 1 ; Zhou, Yanan 1 ; Zhang, Heng 1 ; Wei, Qian 1 ; Luo, Bin 1 ; Xie, Yingge 2 ; Wang, Cheng 1 ; Xue, Xuzhang 1 ; Li, Aixue 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Intelligent Equipment Res Ctr, Beijing 100097, Peoples R China
2.Northwest A&F Univ, Coll Sci, Yangling 712100, Shaanxi, Peoples R China
关键词： Molecularly imprinted polymer; Glycine betaine; In vivo measurement; Electrochemical sensor
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2024 年 435 卷
页码：
收录情况： SCI
摘要： Glycine betaine (GB) is a bioactive molecule protecting plants from abiotic stress. This study fabricated an ultrasensitive molecular imprinted polymer (MIP) electrochemical sensor to perform in vivo measurements of GB. Polydopamine (PDA) was formed on the carboxylated multi-walled carbon nanotubes (COOH-MWCNTs) by spontaneous polymerisation of dopamine (DA). Then MIP-coated MWCNTs were fabricated on a Au nanoparticles (NP) and thionine (Thi) modified screen-printed electrode (SPE). The MIP-COOH-MWCNTs/pThi/ AuNPs/SPE exhibited an ultrasensitive GB detection response between 1 fmol/L and 10 mmol/L (R-2 = 0.996) with a low detection limit (0.707 fmol/L, S/N = 3). In vivo measurement of GB in cucumber seedling leaves under different salinity stress conditions confirmed the practical applicability of the MIP sensor. Thus, this study proposed a novel and promising fabrication method for an electrochemical MIP sensor that has broad application prospects in precision agriculture.
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