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RNA-Seq analysis reveals the important co-expressed genes associated with polyphyllin biosynthesis during the developmental stages of Paris polyphylla

文献类型： 外文期刊

作者： Gao, Xiaoyang 1 ; Su, Qixuan 1 ; Li, Jing 3 ; Yang, Wenjing 1 ; Yao, Baolin 1 ; Guo, Jiawei 5 ; Li, Shengying 5 ; Liu, Changning 1 ;

作者机构： 1.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China

2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China

3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Hainan, Peoples R China

4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

5.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Shandong, Peoples R China

6.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Menglun 666303, Yunnan, Peoples R China

7.Chinese Acad Sci, Innovat Acad Seed Design, Kunming 650223, Yunnan, Peoples R China

关键词： Polyphyllin concentration; Spatiotemporal gene expression; Developmental stage; Polyphyllin biosynthesis; Gene co-expression network

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2022 年 23 卷 SUPPL 1 期

页码：

收录情况： SCI

摘要： Background Plants synthesize metabolites to adapt to a continuously changing environment. Metabolite biosynthesis often occurs in response to the tissue-specific combinatorial developmental cues that are transcriptionally regulated. Polyphyllins are the major bioactive components in Paris species that demonstrate hemostatic, anti-inflammatory and antitumor effects and have considerable market demands. However, the mechanisms underlying polyphyllin biosynthesis and regulation during plant development have not been fully elucidated. Results Tissue samples of P. polyphylla var. yunnanensis during the four dominant developmental stages were collected and investigated using high-performance liquid chromatography and RNA sequencing. Polyphyllin concentrations in the different tissues were found to be highly dynamic across developmental stages. Specifically, decreasing trends in polyphyllin concentration were observed in the aerial vegetative tissues, whereas an increasing trend was observed in the rhizomes. Consistent with the aforementioned polyphyllin concentration trends, different patterns of spatiotemporal gene expression in the vegetative tissues were found to be closely related with polyphyllin biosynthesis. Additionally, molecular dissection of the pathway components revealed 137 candidate genes involved in the upstream pathway of polyphyllin backbone biosynthesis. Furthermore, gene co-expression network analysis revealed 74 transcription factor genes and one transporter gene associated with polyphyllin biosynthesis and allocation. Conclusions Our findings outline the framework for understanding the biosynthesis and accumulation of polyphyllins during plant development and contribute to future research in elucidating the molecular mechanism underlying polyphyllin regulation and accumulation in P. polyphylla.

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