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Analyzing cadmium-phytochelatin2 complexes in plant using terahertz and circular dichroism information

文献类型： 外文期刊

作者： Zhao, Yinglei 1 ; Gouda, Mostafa 2 ; Yu, Guohong 1 ; Zhang, Chenghao 1 ; Lin, Lei 2 ; Nie, Pengcheng 2 ; Huang, Wei 1 ; Y 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agr Equipment, Hangzhou 310000, Peoples R China

2.Zhejiang Univ, Coll Biosyst Engn & Food Sci, 866 Yuhangtang Rd, Hangzhou 310058, Peoples R China

3.Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Hangzhou 310058, Peoples R China

4.Minist Agr, Key Lab Spect Sensing, Hangzhou 310058, Peoples R China

5.Natl Res Ctr, Dept Nutr & Food Sci, Giza, Egypt

关键词： Terahertz spectroscopy; Circular dichroism; Phytochelatin; Cadmium; Complexes

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN： 0147-6513

年卷期： 2021 年 225 卷

页码：

收录情况： SCI

摘要： Phytochelatins are plants' small metal-binding peptides which chelate internal heavy metals to form nontoxic complexes. Detecting the complexes in plants would simplify identification of cultivars with both high tolerance and enrichment capabilities for heavy metals which represent phytoextraction performance. Thus, a terahertz spectroscopy combined with density functional theory, chemometrics and circular dichroism was used for characterization of phytochelatin2 (PC2), Cd-PC2 mixture standards, and pak choi (Brassica chinensis) leaves as a plant model. Results showed PC2 chelates Cd2+ in a 2:1 ratio to form Cd(PC2)(2) complex; Cd connected to thoils of PC2 and changed beta-turn and random coil of PC2 peptide chain to beta-Sheet which presented as terahertz vibrations of PC2 around 1.03 and 1.71 THz being suppressed; the best models for detecting the complex in pak choi were obtained by partial least squares regression modeling combined with successive projections algorithm selection; the models used PC2 as a natural probe for visualizing and quantifying chelated Cd in pak choi leaf and achieved a limit of detection up to 1.151 ppm. This study suggested that terahertz information of the heavy metal-PCs complexes is qualified for representing a simpler alternative to classical index for evaluating phytoextraction performance of plant; it provided a general protocol for structure analysis and detection of heavy metal-PCs complexes in plant by terahertz absorbance.

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