文献类型： 外文期刊

作者： Qiufeng, Y. ¹ ; Zhengrong, W. ¹ ; Kun, G. ¹ ; Yi, C. ¹ ; Wei, W. ² ; Mingzhong, L. ¹ ;

作者机构： 1.Yangtze Univ, Coll Anim Sci, Engn Res Ctr Ecol & Agr Use Wetland, Hubei Key Lab Waterlogging Disaster & Agr Use Wetl, Jingzhou 434025, Peoples R China

2.Zhejiang Acad Agr China, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China

关键词： Whitmania pigra; Soil water content; Overwintering; Survival; Antioxidant; status; Lysozyme

期刊名称：IRANIAN JOURNAL OF FISHERIES SCIENCES （ 影响因子：0.9； 五年影响因子：0.9 ）

ISSN： 1562-2916

年卷期： 2023 年 22 卷 2 期

页码：

收录情况： SCI

摘要： Leech is an important aquatic animal parasite that poses a great threat to the aquaculture industry. However, leech (Whitmania pigra) is an important traditional Chinese medicine and has been widely used to treat cardiovascular or other chronic diseases. Although there has been a growing interest in W. pigra rearing in the South and East of China, the technology of artificial breeding of W. pigra during overwintering is still imperfect. In this study, various soil water contents (30%, 40%, 50%, 60%, and 70%) were tested on W. pigra during a 104-day overwintering period, and their physiological responses were evaluated. During the trial period, the survival rate of W. pigra varied under different soil water contents and the highest and lowest survival rates were 86.67 & PLUSMN;5.77 and 23.33 & PLUSMN;11.55 % at the soil water content of 50 and 70%, respectively. The weight-loss rate of W. pigra decreased when the soil water content increased. The activities of antioxidant enzymes, superoxide dismutase and catalase of the leech at 50% soil water content were lower than those in other groups, however, the highest activities of the immune enzyme, alkaline phosphatase, and lysozyme were obtained in the five groups. These results suggest that soil water content is a key environmental factor that affects W. pigra during overwintering and 50% soil water content is the optimal level for W. pigra during the overwintering period.