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Identification of SNP loci and candidate genes genetically controlling norisoprenoids in grape berry based on genome-wide association study

文献类型： 外文期刊

作者： Sun, Qi 1 ; He, Lei 1 ; Sun, Lei 3 ; Xu, Hai-Ying 3 ; Fu, Ya-Qun 1 ; Sun, Zheng-Yang 1 ; Zhu, Bao-Qing 4 ; Duan, Chang-Qing 1 ; Pan, Qiu-Hong 1 ;

作者机构： 1.China Agr Univ, Coll Food Sci & Nutr Engn, Ctr Viticulture & Enol, Beijing, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Viticulture & Enol, Beijing, Peoples R China

3.Beijing Acad Forestry & Pomol Sci, Beijing, Peoples R China

4.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing, Peoples R China

关键词： grape berry; norisoprenoids; single nucleotide polymorphism; genome-wide association study; genetic regulation

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Obtaining new grapevine varieties with unique aromas has been a long-standing goal of breeders. Norisoprenoids are of particular interest to wine producers and researchers, as these compounds are responsible for the important varietal aromas in wine, characterized by a complex floral and fruity smell, and are likely present in all grape varieties. However, the single-nucleotide polymorphism (SNP) loci and candidate genes genetically controlling the norisoprenoid content in grape berry remain unknown. To this end, in this study, we investigated 13 norisoprenoid traits across two years in an F-1 population consisting of 149 individuals from a hybrid of Vitis vinifera L. cv. Muscat Alexandria and V. vinifera L. cv. Christmas Rose. Based on 568,953 SNP markers, genome-wide association analysis revealed that 27 candidate SNP loci belonging to 18 genes were significantly associated with the concentrations of norisoprenoid components in grape berry. Among them, 13 SNPs were confirmed in a grapevine germplasm population comprising 97 varieties, including two non-synonymous mutations SNPs within the VvDXS1 and VvGGPPS genes, respectively in the isoprenoid metabolic pathway. Genotype analysis showed that the grapevine individuals with the heterozygous genotype C/T at chr5:2987350 of VvGGPPS accumulated higher average levels of 6-methyl-5-hepten-2-one and beta-cyclocitral than those with the homozygous genotype C/C. Furthermore, VvGGPPS was highly expressed in individuals with high norisoprenoids concentrations. Transient overexpression of VvGGPPS in the leaves of Vitis quinquangularis and tobacco resulted in an increase in norisoprenoid concentrations. These findings indicate the importance of VvGGPPS in the genetic control of norisoprenoids in grape berries, serving as a potential molecular breeding target for aroma.

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