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Feasibility and limitations of using specific nitrification inhibitors to differentiate ammonia oxidizer activity

文献类型： 外文期刊

作者： Tan, Che 1 ; Zeng, Yu 1 ; Gubry-Rangin, Cecile 2 ; Yin, Chang 3 ; Liang, Yongchao 1 ;

作者机构： 1.Zhejiang Univ, Coll Environm & Resource Sci, Key Lab Environm Remediat & Ecol Hlth, Minist Educ, Hangzhou 310058, Peoples R China

2.Univ Aberdeen, Sch Biol Sci, Cruickshank Bldg, Aberdeen AB24 3UU, Scotland

3.Zhejiang Acad Agr Sci, Inst Environm Resource Soil & Fertilizer, 298 Desheng Middle Rd, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Ammonia-oxidizing archaea; Ammonia-oxidizing bacteria; Comammox Nitrospira; Nitrification inhibitors; Nitrous oxide; Meta-analysis

期刊名称：SOIL BIOLOGY & BIOCHEMISTRY （ 影响因子：10.3； 五年影响因子：11.5 ）

ISSN： 0038-0717

年卷期： 2025 年 208 卷

页码：

收录情况： SCI

摘要： The application of nitrification inhibitors targeting distinct ammonia-oxidizing guilds-complete ammonia oxidizers (comammox), ammonia-oxidizing archaea (AOA), and bacteria (AOB)-has facilitated elucidating their functional significance across diverse ecosystems. However, the specificity and reliability of these inhibitors remain controversial and have not been thoroughly evaluated. In this study, we first conducted a meta-analysis of several specific nitrification inhibitors: acetylene, 1-octyne, 3,4-dimethylpyrazole phosphate (DMPP), 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), and chlorate, focusing on their effects on AOA, AOB and comammox Nitrospira. Acetylene broadly inhibited the growth of all ammonia oxidizers. Both 1-octyne and higher concentrations (>= 1.5%) of DMPP exhibited dual regulatory effects: inhibiting the growth of AOB and comammox Nitrospira (clade B for 1-octyne; all clades for DMPP) while stimulating AOA growth. PTIO inhibited AOA and AOB but had no significant effect on comammox Nitrospira clade A growth. In contrast, chlorate specifically inhibited comammox Nitrospira growth. To assess the ecological relevance of these synthesized patterns, particularly the guild-specific effects, we conducted microcosm experiments. The results revealed that PTIO failed to inhibit AOA growth in wetland soil. The specificity of 1-octyne and chlorate is dose-dependent. Notably, chlorate at 10 mM completely inhibited the growth of all ammonia oxidizers, while its addition consistently stimulated soil nitrous oxide (N2O) emissions, indicating it cannot reliably differentiate the contribution of different nitrifier guilds to N2O emissions. These findings collectively challenge the conventional use of inhibitor-based assays, underscoring the necessity to evaluate the effectiveness of inhibitors in distinguishing the functional importance of ammonia oxidizers.

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