Blue light receptor cryptochrome determines primordia formation, fruiting body development and pileus opening in macro mushroom Flammulina filiformis
文献类型： 外文期刊
作者： Wei, Shiyun 1 ; Jia, Hui 1 ; Zhao, Honglei 1 ; Ju, Huiming 1 ; Li, Hui 4 ; Xu, Chang 1 ; Liu, Bo 1 ; Hong, Yanhong 1 ; Fang, Yina 1 ; Li, Ziyan 1 ; Niu, Mengyao 1 ; Yao, Sen 4 ; Tao, Yongxin 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Hort, 15 Shangxiadian Rd, Fuzhou 350002, Fujian, Peoples R China
2.Fujian Agr & Forestry Univ, Key Lab Minist Educ Genet Breeding & Multiple Util, Fuzhou 350002, Fujian, Peoples R China
3.Fujian Agr & Forestry Univ, Mycol Res Ctr, Fuzhou 350002, Fujian, Peoples R China
4.Hebei Acad Agr & Forestry Sci, Inst Cash Crops, Shijiazhuang 050051, Hebei, Peoples R China
关键词： Flammulina filiformis; Cryptochrome; Stipe elongation; Pileus opening; Blue light; Cell wall
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.2； 五年影响因子：4.6 ）
ISSN： 0304-4238
年卷期： 2025 年 339 卷
页码：
收录情况： SCI
摘要： Blue light, as a crucial environmental factor, can regulate the morphological development of macro fungi. The receptor WCC (white collar complex) has been found to play a role in controlling fruiting body elongation, but another important receptor, cryptochrome, was easily overlooked its important role. In this study, we investigated the overall impacts of cryptochrome gene ( FfCry ) knockdown on the morphological development of fruiting bodies and its downstream regulation of metabolic activities in Flammulina filiformis. Two knockdown strains FfCry-kd8 and FfCry-kd13 were constructed to explore the morphological changes after FfCry knockdown. The results showed that the number of primordia of FfCry-kd8 and FfCry-kd13 was reduced by 33.6 % and 38.0 %. Obviously, FfCry-kd8 and FfCry-kd13 showed much shorter and finer stipes (With stipe lengths reduced by 51.8 % and 55.1 %, and stipe diameters by 43.4 % and 34.6 %), compared with wild type (WT). Especially, two FfCry-kd transformants showed much smaller pileus than WT (diameter was decreased by 52.6 % and 55.9 %), meaning that FfCry knockdown inhibited the pileus opening. RNA Sequencing (RNA-Seq) analysis identified that 216 genes were up-regulated and 164 genes were down-regulated after FfCry knockdown. The FfCry gene knockdown primarily activated the glucan 1,3-beta-glucosidase, peptide receptor, and beta- alanine metabolic pathways; while mainly inhibiting starch and sucrose metabolism, as well as various N-polymer biosynthesis and sphingolipid metabolism, etc. These metabolic pathways played important roles in the formation of cell walls and the fluidity of cell membranes. The results clearly demonstrate the significant changes in the fruiting body morphology of F. filiformis in the case of partial loss of FfCry function. This finding holds guiding significance for the scientific utilization of blue light to regulate the commercial characteristics of mushrooms.
- 相关文献
作者其他论文 更多>>
-
Identification of Thalictrum squarrosum as an alternate host for Puccinia triticina and pathogen analysis of Thalictrum squarrosum rust
作者：Zhao, Na;Huang, Liang;Ren, Jun;Yi, Ting;Li, Hongfu;Zhang, Hao;Liu, Bo;Gao, Li;Chen, Wanquan;Liu, Taiguo;Zhao, Na;Huang, Liang;Ren, Jun;Yi, Ting;Li, Hongfu;Zhang, Hao;Gao, Li;Chen, Wanquan;Liu, Taiguo;Zhang, Mengya;Yan, Hongfei;Liu, Taiguo
关键词：wheat leaf rust;
Puccinia triticina ;Thalictrum squarrosum ; alternate host; sexual reproduction -
Integrated transcriptome and metabolome analysis revealed differential drought stress response mechanisms of wheat seedlings with varying drought tolerance
作者：Guo, Xiaorui;Lv, Liangjie;Zhao, Aiju;Zhao, Wei;Liu, Yuping;Li, Zetong;Li, Hui;Chen, Xiyong
关键词：
Triticum aestivum L .; Drought stress; Metabolome; Transcriptome; Flavonoids; Phenolic acids -
Pleiotropic Quantitative Trait Loci (QTL) Mining for Regulating Wheat Processing Quality- and Yield-Related Traits
作者：Zhao, Jie;Sun, Lijing;Hu, Mengyun;Liu, Qian;Xu, Junjie;Yang, Jing;Wang, Peinan;Li, Qianying;Li, Hui;Zhang, Yingjun;Mu, Liming;Wang, Jianbing
关键词：wheat; GWAS; processing quality; grain yield; pleiotropic QTL
-
Light and phytochrome PHY control the production of edible fungus Flammulina filiformis by regulating the morphogenesis of fruiting bodies and L-lysine accumulation
作者：Chen, Yizhao;Ju, Huimin;Xu, Chang;Jia, Hui;Xian, Lijun;Yuan, Chengjin;Guo, Zexuan;Zhang, Xijin;Yu, Yilin;Tao, Yongxin;Chen, Yizhao;Ju, Huimin;Xu, Chang;Jia, Hui;Xian, Lijun;Yuan, Chengjin;Guo, Zexuan;Zhang, Xijin;Yu, Yilin;Tao, Yongxin;Chen, Yizhao;Ju, Huimin;Xu, Chang;Jia, Hui;Xian, Lijun;Yuan, Chengjin;Guo, Zexuan;Zhang, Xijin;Yu, Yilin;Tao, Yongxin;Li, Hui
关键词：Flammulina filiformis; Phytochrome; Cell division; Cell wall remodeling; L -lysine catabolism; Stipe elongation
-
Targeted metabolome and transcriptome analyses reveal changes in gibberellin and related cell wall-acting enzyme-encoding genes during stipe elongation in Flammulina filiformis
作者：Li, Hui;Yao, Sen;Xia, Weiwei;Ma, Xinbin;Shi, Lei;Ju, Huimin;Li, Ziyan;Zhong, Yingli;Tao, Yongxin;Shi, Lei;Ju, Huimin;Li, Ziyan;Zhong, Yingli;Xie, Baogui;Tao, Yongxin
关键词：Flammulina filiformis; gibberellin; stipe elongation; targeted metabolome; transcriptome
-
Soil Microbial Communities Show Different Patterns under Different Land Use Types in the Coastal Area of Nantong, China
作者：Li, Jinbiao;Hu, Anyong;Zhao, Chuang;Jin, Jiarui;Han, Yujie;Liu, Bo;Li, Jinbiao;Liu, Guangming;Wang, Xiuping
关键词：land-use type; soil properties; microbial diversity and composition; microbial ecological functions
-
Combined analysis of metabolome and transcriptome of wheat kernels reveals constitutive defense mechanism against maize weevils
作者：Lv, Liangjie;Guo, Xiaorui;Zhao, Aiju;Liu, Yuping;Li, Hui;Chen, Xiyong
关键词：Triticum aestivum L; Sitophilus zeamais; metabolome; transcriptome; constitutive defense; flavonoids biosynthesis
