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Nitric oxide synthase-dependent nitric oxide production enhances chilling tolerance of walnut shoots in vitro via involvement chlorophyll fluorescence and other physiological parameter levels

文献类型： 外文期刊

作者： Dong, Ningguang 1 ; Li, Yuanfa 2 ; Qi, Jianxun 1 ; Chen, Yonghao 1 ; Hao, Yanbin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing Engn Res Ctr Deciduous Fruit Trees, Key Lab Urban Agr North China,Minist Agr, Beijing 100093, Peoples R China

2.Chinese Acad Forestry, Res Inst Forestry, Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing 100091, Peoples R China

关键词： Nitric oxide;Nitric oxide synthase;Chilling;Juglans regia;Chlorophyll fluorescence

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2018 年 230 卷

页码：

收录情况： SCI

摘要： The role of nitric oxide (NO) in response to chilling stress in walnut (Juglans regia L.) seedlings was investigated. Walnut shoots were treated in vitro with sodium nitroprusside (SNP), NO scavenger 2-phenyl-4,4,5,5-tetramethyl-imidazoline-l-oxy1-3-oxide (PTIO), NO synthase (NOS) inhibitor N-nitro-L-Arg-methyl ester (L-NAME), and nitrate reductase (NR) inhibitor sodium tungstate. Their effects on chilling tolerance, the contents of soluble sugar, proline, and total phenol, reduced glutathione (GSH) and redox state (GSH/GSSG), and the activities of NOS and NR and NO level were analyzed. The results showed that low temperatures at 4 C induced electrolyte leakage, lipid peroxidation and photosynthetic efficiency suppression, which were dramatically alleviated by exogenous application of SNP. The levels of soluble sugar, proline, total phenol, GSH and GSH/GSSG increased evidently in the presence of SNP under chilling stress. The above protective effect of SNP could be reversed by NO scavenger PTIO or NOS inhibitor. However, NR inhibitors did not affect the protective effect of SNP. Moreover, measurements of NOS activity and NO production showed that both NOS activity and endogenous NO content increased markedly under chilling stress. The NO production was markedly reduced by NOS inhibitors, but not affected by NR inhibitors in the presence or absence of chilling stress. Taken together, these results suggest that NOS-dependent NO production may confer chilling tolerance in walnut shoots in vitro by enhancing the levels of soluble sugar, proline, total phenol, GSH and GSH/GSSG to alleviate electrolyte leakage, lipid peroxidation and photosynthetic efficiency suppression induced by chilling stress.

  • 相关文献

[1]Effects of Abscisic Acid and Nitric Oxide on Chilling Resistance and Activation of the Antioxidant System in Walnut Shoots In Vitro. Dong, Ningguang,Qi, Jianxun,Chen, Yonghao,Hao, Yanbin,Li, Yuanfa.

[2]Tomato seeds maturity detection system based on chlorophyll fluorescence. Li, Cuiling,Wang, Xiu,Meng, Zhijun,Li, Cuiling,Wang, Xiu,Meng, Zhijun. 2016

[3]Phytotoxicity of atrazine to emergent hydrophyte, Iris pseudacorus L.. Wang, Qinghai,Li, Cui,Xiao, Bo,Que, Xiaoe.

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