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Molecular cloning, expression analysis, and subcellular localization of FLOWERING LOCUS T (FT) in carrot (Daucus carota L.)

文献类型： 外文期刊

作者： Zhan, Zongxiang 1 ; Zhang, Chunsha 1 ; Zhang, Hongwei 1 ; Li, Xiaojie 1 ; Wen, Changlong 1 ; Liang, Yi 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China

3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei,

关键词： Carrot;Floral transition;Quantitative RT-PCR;Subcellular localization

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2017 年 37 卷 12 期

页码：

收录情况： SCI

摘要： Flowering, an important agronomic trait for seed plants, represents the end of vegetative growth and begins to reproduce. In regulating the floral transition, FLOWERING LOCUS T (FT) encoding a mobile floral signal protein that belongs to the phosphatidylethanolamine-binding protein (PEBP) family acts as critical role. Here, an FT-like gene, DcFT, was isolated and cloned from European Daucus carota L. cultivar "Nantes-H06" (GenBank accession number KY768910), and an alignment of the DcFT protein and other FT-homolog proteins showed that it shared 88.00% similarity with CsFT from Camellia sinensis. Phylogenetic tree analysis indicated that DcFT had the closest relationship with GpFT (Gypsophila paniculata). Quantitative RT-PCR was analyzed to show that the expression pattern of DcFT in inflorescences sharply increased after 10DAA and then slowly increased reaching the maximum at 30DAA. Subcellular localization made clear that the DcFT protein was located in the nucleus and cytoplasm. Broadly speaking, DcFT is an FT-like homologous gene in carrot regulating the floral transition and could be a candidate gene for accelerating the process in carrot breeding.

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