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Influence of pCP1NetB ancillary genes on the virulence of Clostridium perfringens poultry necrotic enteritis strain CP1

文献类型： 外文期刊

作者： Zhou, Hongzhuan 1 ; Lepp, Dion 2 ; Pei, Yanlong 3 ; Liu, Mei 2 ; Yin, Xianhua 2 ; Ma, Rongcai 1 ; Prescott, John F. 3 ; G 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China

2.Agr & Agri Food Canada, Guelph Res & Dev Ctr, 93 Stone Rd West, Guelph, ON N1G 5C9, Canada

3.Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada

关键词： Necrotic enteritis;Clostridium perfringens;Plasmid-deficient mutant;NELoc-1;NetB

期刊名称：GUT PATHOGENS （ 影响因子：4.181； 五年影响因子：4.524 ）

ISSN： 1757-4749

年卷期： 2017 年 9 卷

页码：

收录情况： SCI

摘要： Background: Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. The NetB toxin plays a critical role in the pathogenesis of NE. We previously demonstrated that netB is located within a 42 kb plasmid-encoded pathogenicity locus (NELoc-1), which also encodes 36 additional genes. Although NetB clearly plays a role in pathogenesis, the involvement of the other NELoc-1 genes has not yet been established. The current study was to provide experimental evidence to confirm the involvement of these genes in NE pathogenesis. Results: The present study has characterized a virulent C. perfringens strain (CP1) that has spontaneously lost the NELoc-1-encoding plasmid, pCP1netB. When assessed for cytotoxicity on Leghorn Male Hepatoma (LMH) cells, the culture supernatant of the pCP1netB-deficient CP1 variant (CP1 Delta pCP1netB) demonstrated significantly reduced cytotoxicity compared to the wild-type. In addition, CP1 Delta pCP1netB was unable to cause intestinal lesions in chickens in a NE disease model. When netB alone was introduced into CP1 Delta pCP1netB, in vitro cytotoxicity was restored to the wild-type level; however, it did not completely restore virulence when used to challenge broiler chickens [ mean lesion score of 0.71 compared to 3.23 in the wild type control group (n = 14)]. Conclusions: The results of this study suggest that other genes present in NELoc-1, in addition to netB, are required for full virulence in the chicken challenge model.

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[1]The Agr-Like Quorum Sensing System Is Required for Pathogenesis of Necrotic Enteritis Caused by Clostridium perfringens in Poultry. Yu, Qiang,Nie, Shao-Ping,Xie, Ming-Yong,Yu, Qiang,Lepp, Dion,Wu, Tao,Zhou, Hongzhuan,Yin, Xianhua,Yu, Hai,Gong, Joshua,Gohari, Iman Mehdizadeh,Prescott, John F.,Wu, Tao,Zhou, Hongzhuan.

[2]Necrotic enteritis locus 1 diguanylate cyclase and phosphodiesterase (cyclic-di-GMP) gene mutation attenuates virulence in an avian necrotic enteritis isolate of Clostridium perfringens. Parreira, Valeria R.,Ojha, Shivani,Gohari, Iman Mehdizadeh,Susta, Leonardo,Prescott, John F.,Lepp, Dion,Zhou, Hongzhuan,Gong, Jianhua,Zhou, Hongzhuan. 2017

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