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Transcriptome Analysis of Pepper (Capsicum annuum) Revealed a Role of 24-Epibrassinolide in Response to Chilling

文献类型： 外文期刊

作者： Li, Jie 1 ; Yang, Ping 2 ; Kang, Jungen 3 ; Gan, Yantai 4 ; Yu, Jihua 1 ; Calderon-Urrea, Alejandro 6 ; Lyu, Jian 1 ; Zha 1 ;

作者机构： 1.Gansu Agr Univ, Coll Hort, Dept Facil Hort Sci, Lanzhou, Peoples R China

2.Gansu Agr Univ, Coll Agron, Dept Crop Cultivat & Farming Syst, Lanzhou, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Dept Vegetable Genet & Breeding, Beijing, Peoples R China

4.Agr & Agri Food Canada, Semiarid Prairie Agr Res Ctr, Swift Current, SK, Canada

5.Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou, Peoples R China

6.Calif State Univ Fresno, Dept Biol, Fresno, CA 93740 USA

关键词： pepper;Brassinosteroid;chill-stress;transcriptome;RNA sequencing

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Brassinosteroids (BRs) have positive effects on many processes during plant growth, development, and various abiotic stress responses. However, little information is available regarding the global gene expression of BRs in response to chilling stress in pepper. In this study, we used RNA sequencing to determine the molecular roles of 24-epibrassinolide (EBR) during a chilling stress response. There were 39,829 transcripts, and, among them, 656 were differently expressed genes (DEGs) following EBR treatment (Chill+EBR) compared with the control (Chill only), including 335 up regulated and 321 down-regulated DEGs. We selected 20 genes out of the 656 DEGs for RT-qPCR analysis to confirm the RNA-Seq. Based on GO enrich and KEGG pathway analysis, we found that photosynthesis was significantly up-enriched in biological processes, accompanied by significant increases in the net photosynthetic rate (Pn). Fv/Fm, and chlorophyll content. Furthermore, the results indicate that EBR enhanced endogenous levels of salicylic acid (SA) and jasmonic acid (JA) while suppressing the ethylene (ETH) biosynthesis pathway, suggesting that BRs function via a synergistic cross-talk with SA, JA, and ETH signaling pathways in response to chilling stress. In addition, EBR induced cellulose synthase-like protein and UDP-glycosyltransferase, suggesting a contribution to the formation of cell wall and hormone metabolism. EBR also triggered the calcium signaling transduction in cytoplasm, and activated the expression of cellular redox homeostasis related genes, such as GSTX1, PER72, and CAT2. This work, therefor, identified the specific genes showed different expression patterns in EBR-treated pepper and associated with the processes of hormone metabolism, redox, signaling, transcription, and defense. Our study provides the first evidence of the potent roles of BRs, at the transcription level, to induce the tolerance to chilling stress in pepper as a function of the combination of the transcriptional activities, signaling transduction, and metabolic homeostasis.

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