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Identification and Expression Analysis of a Novel HbCIPK2-Interacting Ferredoxin from Halophyte H. brevisubulatum

文献类型： 外文期刊

作者： Zhang, Chao 1 ; Ge, Rongchao 2 ; Zhang, Junwen 3 ; Chen, Yajuan 1 ; Wang, Hongzhi 1 ; Wei, Jianhua 1 ; Li, Ruifen 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing, Peoples R China

2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang, Peoples R China

3.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100005, Peoples R China

4.Peking Union Med Coll, Beijing 100005, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 12 期

页码：

收录情况： SCI

摘要： Ferredoxin is a small iron-sulfer protein involved in various one-eletron transfer pathways. Little is known about how ferredoxin is regulated to distribute electron under abiotic stress. Our previous study has showed that HbCIPK2 conferred salinity and drought tolerance. Thus, we hypothesized that HbCIPK2 could mediate the activities of interacting partners as a signal transducer. In this report, we identified a novel HbCIPK2-interacting ferredoxin (HbFd1) from halophyte Hordeum brevisubulatum by yeast two-hybrid screens, confirmed this interaction by BiFC in vivo and CoIP in vitro, and presented the expression pattern of HbFd1. HbFd1 was down-regulated under salinity and cold stress but up-regulated under PEG stress, its expression showed tissue-specific, mainly in shoot chloroplast, belonging to leaf-type subgroup. Moreover, HbCIPK2 could recruit HbFd1 to the nucleus for their interaction. The C-terminal segment in HbFd1 protein was involved in the interaction with HbCIPK2. These results provided insight into the connection between CBL-CIPK signaling network and Fd-dependent metabolic pathways.

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