Coat protein-mediated resistance to Turnip mosaic virus in Chinese cabbage (B. rapa ssp pekinensis) by the pollen-tube pathway
文献类型： 外文期刊
作者： Wei, Han Li 1 ; Yu, Ge 1 ; Lei, Rao 2 ; Shi, Cui Chong 1 ; Ping, Qu Shu 1 ;
作者机构： 1.Northeast Agr Univ, Dept Hort, Key Lab Biol & Genet Improvement Hort Crops North, Harbin 150030, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agr Biotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Chinese cabbage (B. rapa ssp pekinensis);Turnip mosaic virus;TuMV-CP;Coat protein-mediated resistance;Pollen-tube pathway
期刊名称：RESEARCH JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.233； 五年影响因子：0.252 ）
ISSN： 0973-6263
年卷期： 2014 年 9 卷 2 期
页码：
收录情况： SCI
摘要： In this research we established a plant transformation system of coat protein-mediated resistance to Turnip mosaic virus. The gene TuMV-CP was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid pBBBast-TuMV-CP (9.79 kb) which was composed of the gene TuMV-CP, expression regulatory sequence (CaMV 35S promoter, OCS terminator), the herbicide resistant gene Bar and other elements. A total of 0.15% of T-0 plants was positive for the gene TuMV-CP by specific PCR. Further PCR Southern blot analyses showed that TuMV-CP insertions were harbored in T-0 plants.
- 相关文献
