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Forming coumarin C-glycosides via biocatalysis: Characterization of a C-glycosyltransferase from Angelica decursiva

文献类型： 外文期刊

作者： Wang, Zhiming 1 ; He, Yuedong 3 ; Liao, Lijing 4 ; Zhang, Yuhao 5 ; Zhao, Yucheng 5 ; Xiao, Yibei 4 ; Jiang, Xuefei 1 ; Qiao, Fei 6 ;

作者机构： 1.Hainan Univ HNU, Coll Hort, Key Lab Qual Regulat Trop Hort Plants Hainan Prov, Haikou, Hainan, Peoples R China

2.Hainan Univ HNU, Coll Hort, Key Lab Trop Agritourism Greenhouse Haikou, Haikou, Hainan, Peoples R China

3.Hunan Agr Univ, Coll Hort, Changsha, Hunan, Peoples R China

4.China Pharmaceut Univ, Sch Pharm, Nanjing, Jiangsu, Peoples R China

5.China Pharmaceut Univ, Dept Nat Med Chem, State Key Lab Nat Med, Nanjing, Peoples R China

6.Chinese Acad Trop Agr Sci, Minist Agr, Key Lab Crop Gene Resources & Germplasm Enhanceme, Haikou, Hainan, Peoples R China

关键词： Angelica decursiva; C-glycosyltransferase; Coumarin; Catalytic mechanism; Molecular docking

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.322； 五年影响因子：3.498 ）

ISSN： 0006-291X

年卷期： 2022 年 614 卷

页码：

收录情况： SCI

摘要： A glycosyl transferase, isolated from Angelica decursiva a medical herb rich in coumarin, shows C-glycosyl transferase activity by in vitro activity assay using 5,7-dihydroxycoumarin as substrate, producing a C-glycosylated product at position C ' 8 along with the main product at C ' 6 position. Catalytic promiscuity assay shows that AdCGT also displays O- or C-glycosylation activity to other coumarins and flavonoids. When phloretin and 2,4,6-trihydroxyacetophenone were fed as substrates, AdCGT catalyzed the formation of di-C-glycosides. Therefore, AdCGT is a multifunctional glycosyltransferase with a broad substrate acceptability. This work highlights the potential of AdCGT as a catalyst for glycosylation of coumarin and reveals a new regio-selective C-glycosyltransferase, providing a basis for exploring the mechanism of coumarin glycosylation. (c) 2022 Elsevier Inc. All rights reserved.

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