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SlnM gene overexpression with different promoters on natamycin production in Streptomyces lydicus A02

文献类型： 外文期刊

作者： Wu, Huiling 1 ; Liu, Weicheng 1 ; Dong, Dan 1 ; Li, Jinjin 1 ; Zhang, Dianpeng 1 ; Lu, Caige 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

关键词： Dual promoters;Natamycin yield;Pathway-specific regulatory gene;SlnM gene;Streptomyces lydicus

期刊名称：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.346； 五年影响因子：3.426 ）

ISSN： 1367-5435

年卷期： 2014 年 41 卷 1 期

页码：

收录情况： SCI

摘要： Natamycin is an important polyene macrolide antifungal agent produced by several Streptomyces strains and is widely used as a food preservative and fungicide in food, medicinal and veterinary products. In order to increase the yield of natamycin, this study aimed at cloning and overexpressing a natamycin-positive regulator, slnM2, with different promoters in the newly isolated strain Streptomyces lydicus A02, which is capable of producing natamycin. The slnM gene in S. lydicus is highly similar to gene pimM (scnRII), the pathway-specific positive regulator of natamycin biosynthesis in S. natalensis and S. chattanoogensis, which are PAS-LuxR regulators. Three engineered strains of S. lydicus, AM01, AM02 and AM03, were generated by inserting an additional copy of slnM2 with an ermEp* promoter, inserting an additional copy of slnM2 with dual promoters, ermEp* and its own promoter, and inserting an additional copy of slnM2 with its own promoter, respectively. No obvious changes in growth were observed between the engineered and wild-type strains. However, natamycin production in the engineered strains was significantly enhanced, by 2.4-fold in strain AM01, 3.0-fold in strain AM02 and 1.9-fold in strain AM03 when compared to the strain A02 in YEME medium without sucrose. These results indicated that the ermEp* promoter was more active than the native promoter of slnM2. Overall, dual promoters displayed the highest transcription of biosynthetic genes and yield of natamycin.

  • 相关文献

[1]IDENTIFICATION OF AN ANTIFUNGAL METABOLITE PRODUCED BY A POTENTIAL BIOCONTROL ACTINOMYCES STRAIN A01. Lu, Cai Ge,Liu, Wei Cheng,Qiu, Ji Yan,Liu, Ting,Liu, De Wen,Lu, Cai Ge,Wang, Hui Min. 2008

[2]Efficient transformation and expression of the glucanase gene from Bacillus megaterium in the biocontrol strain Streptomyces lydicus A02. Wu, Huiling,Dong, Dan,Li, Jinjin,Liu, Weicheng,Liu, Ting,Zhang, Taotao,Tian, Zhaofeng. 2014

[3]Expression of Paenibacillus polymyxa beta-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea. Li, Jinjin,Liu, Weicheng,Dong, Dan,Liu, Ting,Zhang, Taotao,Lu, Caige,Liu, Dewen,Zhang, Dianpeng,Wu, Huiling,Luo, Lijin.

[4]Optimization of the Fermentation Process for Streptomyces lydicus A02 Producing Natamycin. Weicheng Liu,Ting Liu,Caige Lu,Taotao Zhang,Dewen Liu,Yupeng Hua. 2012

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