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Cloning and characterization of the pyrG gene of Pleurotus ostreatus and Pleurotus eryngii

文献类型： 外文期刊

作者： Yin, Yonggang 1 ; Liu, Yu 2 ; Wang, Shouxian 2 ; Li, Huamin 3 ; Niu, Liying 2 ; Zhao, Shuang 2 ; Geng, Xiaoli 2 ; Li, Min 1 ;

作者机构： 1.Agr Univ Hebei, Coll Hort, Baoding 071001, Hebei, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

3.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China

关键词： Pleurotus ostreatus;Pleurotus eryngii;pyrG;nested PCR;chromosome walking

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2012 年 6 卷 12 期

页码：

收录情况： SCI

摘要： Pleurotus spp. are well known and important cultivated mushrooms. However, the pyrG gene in Pleurotus spp., which is used as a bio-safe selective marker in transformation systems, has not yet been characterized. In the present study, nested degenerated PCR was used to clone conserved fragments of the pyrG gene from Pleurotus ostreatus and Pleurotus eryngii. Chromosome walking technology was then used to obtain the full-length DNA sequence of pyrG. Finally, reverse transcriptase (RT) PCR was used to obtain the pyrG cDNA sequence. The cDNA sequence of P. ostreatus and P. eryngii were all 813 bp in length and encoded 246 amino acids. Comparison of the DNA sequences with cDNA sequences of both P. ostreatus and P. eryngii indicated that pyrG genes of these two strains consisted of two introns and their deduced amino acid sequence showed 93.09% similarity. Moreover, the 5'-flanking region of these two genes was analyzed.

  • 相关文献

[1]Evaluation of genetic diversity of Chinese Pleurotus ostreatus cultivars using DNA sequencing technology. Liu, Yu,Wang, Shouxian,Yin, Yonggang,Xu, Feng.

[2]The spent mushroom substrates of Hypsizigus marmoreus can be an effective component for growing the oyster mushroom Pleurotus ostreatus. Wang, Shouxian,Xu, Feng,Zhao, Shuang,Song, Shuang,Rong, Chengbo,Geng, Xiaoli,Liu, Yu,Wang, Shouxian,Liu, Yu,Li, Zhiming.

[3]Pantoea beijingensis sp nov., isolated from the fruiting body of Pleurotus eryngii. Liu, Yu,Wang, Shouxian,Zhang, Dianpeng,Wei, Shujun,Zhao, Shuang,Xu, Feng,Chen, Sanfeng. 2013

[4]DEVELOPMENT OF A SPECIES-SPECIFIC REAL-TIME PCR ASSAY FOR DIAGNOSING PANTOEA BEIJINGENSIS. Ma, Y.,Rong, C.,Wang, S.,Liu, Y.,Wang, J.,Xu, F.,Ma, Y..

[5]Polyethylene glycol-mediated transformation of fused egfp-hph gene under the control of gpd promoter in Pleurotus eryngii. Yin, Yonggang,Liu, Yu,Wang, Shouxian,Zhao, Shuang,Geng, Xiaoli,Xu, Feng,Yin, Yonggang,Li, Ming,Jin, Haojie.

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