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A semi-fertile interspecific hybrid of Brassica rapa and B. nigra and the cytogenetic analysis of its progeny

文献类型： 外文期刊

作者： Sheng, Xiaoguang 1 ; Wen, Guiju 1 ; Guo, Yangdong 3 ; Yan, Hong 4 ; Zhao, Hong 1 ; Liu, Fan 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Vegetable Sci, Hangzhou 310021, Zhejiang, Peoples R China

3.China Agr Univ, Coll Agron & Biotechnol, Beijing 100094, Peoples R China

4.Beijing Acad Agr Sci, Inst Plant Protect, Beijing 100097, Peoples R China

关键词： Brassica rapa;B. nigra;GISH analysis;Xcc. resistance

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN： 0925-9864

年卷期： 2012 年 59 卷 1 期

页码：

收录情况： SCI

摘要： Black rot is a bacterial disease of Brassica rapa caused by Xanthomonas campestris pv. campestris (Xcc.). Sources of resistance to this disease within B. rapa are insufficient and control measures are limited, making the development of resistant breeding lines extremely important. Certain lines of B. nigra exhibit very high resistance to Xcc. For this study, an interspecific cross between Brassica rapa and B. nigra was performed, and a total of 6 F(1) hybrids were obtained through ovary culture. Five plants (H(1-5)) were relatively slow-growing, entirely sterile, and had 18 chromosomes in the majority of pollen mother cells (PMCs). GISH analysis showed that most of the PMCs had 8 B. nigra chromosomes, which indicated the expected AB genomic constitution. The last plant (H(6)) was partially fertile and the majority of PMCs contained 10 chromosomes of B. rapa and 16 chromosomes of B. nigra, indicating an ABB genomic constitution. Ovary culture techniques were not necessary for the development of the first- or second-backcross generation. Sequence-related amplified polymorphism analysis of F(1), BC(1), and BC(2) plants indicated that some fragments from B. nigra were lost, particularly in the genome of BC(1)/BC(2) in successive generation(s). The BC(2) plants expressing good resistance to Xcc. were observed.

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[1]Initiation and development of microspore embryogenesis and plant regeneration of Brassica nigra. Gu, Honghui,Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng.

[2]Rapid alterations of DNA sequence and cytosine methylation induced by somatic hybridization between Brassica oleracea L. var. italica and Brassica nigra (L.) Koch. Sheng, Xiaoguang,Zhao, Zhenqing,Yu, Huifang,Wang, Jiansheng,Gu, Honghui.

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