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Establishment and Application of Ty-2 Molecular Marker in Tomatoes

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文献类型：外文期刊

作者： Yang, Ruixing ¹ ; Lv, Shuwen ² ; Chai, Min ³ ; Li, Haitao ¹ ;

作者机构： 1.Shenyang Agr Univ, Dept Hort, Dongling Rd 120, Shenyang 110161, Peoples R China

2.Liaoning Acad Agr Sci, Inst Vegetable, Shenyang 110161, Liaoning, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China

关键词： Tomato;Ty-2;Molecular marker;Assisted selection;Tomato disease resistance breeding

期刊名称：THIRD CONFERENCE ON HORTICULTURE SCIENCE AND TECHNOLOGY (CHST 2012)

ISSN：

年卷期： 2012 年

页码：

收录情况： SCI

摘要： In order to obtain molecular marker of resistant gene Ty-2 in tomatoes (Lycopersicon esculentum Mill.), 4 resistant homozygous lines (Ty-2/Ty-2) and 4 susceptible lines (ty-2/ty-2) were amplified by specific primers. The PCR fragments for the resistant homozygous and susceptible lines are 900 bp and 800 bp long, respectively. No Ty-2 resistant gene in Defoe F2, which show resistance to the TYLCV in field. Meanwhile, Only 1 line has the Ty-2 resistant homozygous gene, 5 hybrid lines, and the remaining 26 lines have no Ty-2 resistant gene in the 32 unknown genotype lines. This marker, which can distinguish resistant and susceptible lines, is a co-dominant marker tightly linked to Ty-2 gene. By repeated verification, this marker can be used in assisted selection of tomato disease resistance breeding.

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Establishment and Application of Ty-2 Molecular Marker in Tomatoes

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