文献类型： 外文期刊
作者： Chen, Xuqing 1 ; Hu, Zongli 1 ; Yang, Yuxin 1 ; Pan, Yu 1 ; Zhang, Ning 1 ; Chen, Guoping 1 ;
作者机构： 1.Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Bioengn Coll, Chongqing 400044, Peoples R China
2.Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Human chemokine receptor CCR-5;fission yeast;membrane protein expression;affinity chromatography
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 74 期
页码：
收录情况： SCI
摘要： The human CCR5, a subfamily of G-protein-coupled, seven-transmembrane-domain cell-surface chemokine receptors that binds to beta-chemokine, was heterologously expressed in fission yeast, Schizosaccharomyces pombe, through CCR5-GST fusion protein. The CCR5-GST fusion protein was driven by the full-length nmt1 promoter (Pnmt1) derived from S. pombe. The transcription level of CCR5-GST fusion protein was very high when induced by deprivation of thiamine in the media. About 200 mu g of highly purified CCR5-GST fusion protein was obtained from 3 g of wet cell paste (one liter of cell culture). The SDS-PAGE and western blot analysis indicated that the human membrane protein was efficiently expressed and purified in fission yeast.
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