An infectious clone of the highly pathogenic porcine reproductive and respiratory syndrome virus: Topology of glycoprotein 3 (GP3) addressing the intrachain disulfide bonds
文献类型： 外文期刊
作者： Feng ChunYan 1 ; Liu YueHuan 3 ; Yan JingHua 1 ; Gao, George F. 1 ;
作者机构： 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol CASPMI, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China
4.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China
关键词： porcine reproductive and respiratory syndrome virus;reverse genetic methods;infectious clone;glycoprotein 3;topology
期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）
ISSN： 1001-6538
年卷期： 2011 年 56 卷 26 期
页码：
收录情况： SCI
摘要： The highly pathogenic porcine reproductive and respiratory virus (hpPRRSV) with discontinuous 30 amino acid (aa) deletion as a gene marker has caused great economic loss in pig industry and since 2007 has become the dominant strain prevalent in China and Vietnam since 2007. Reverse genetics method is a powerful tool urgently needed to be used on the hpPRRSV to study the intriguing molecular mechanism of transcription, replication and the virulence determinant factors. In our study, we successfully constructed a full-length infectious clone, prBJSY07, based on hpPRRSV isolate, BJSY07. The rescued virus, vrBJSY07, showed similar growth characters to those of the parental virus, BJSY07. We also found that a rescued virus vBJSY07 generated from pBJSY07 was viable but displayed decreased and delayed reproductive capacity, which might be caused by two amino acids mutations, S83C and S117C in glycoprotein 3 (GP3), acquired during the preparation of the infectious clone. The topology of the wild type GP3 and the mutant were further analyzed by bioinformatics and revealed that the mutated GP3 possessed slightly altered structure, most likely by forming a new disulfide bond between the two new cysteine residues. As GP3 is a cysteine-rich glycoprotein, common for viral glycoproteins, our results show that GP3 can accommodate even more cysteine mutations. Based on this, a topological model of GP3 is proposed by addressing the intrachain disulfides.
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