Characterization of CENH3 proteins and centromere-associated DNA sequences in diploid and allotetraploid Brassica species
文献类型： 外文期刊
作者： Wang, Guixiang 1 ; He, Qunyan 1 ; Liu, Fan 2 ; Cheng, Zhukuan 3 ; Talbert, Paul B. 5 ; Jin, Weiwei 1 ;
作者机构： 1.China Agr Univ, Natl Maize Improvement Ctr China, Key Lab Crop Genet Improvement & Genome, Minist Agr,Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
3.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
5.Fred Hutchinson Canc Res Ctr, Howard Hughes Med Inst, Seat
期刊名称：CHROMOSOMA （ 影响因子：4.316； 五年影响因子：4.159 ）
ISSN： 0009-5915
年卷期： 2011 年 120 卷 4 期
页码：
收录情况： SCI
摘要： CENH3 is a centromere-specific histone H3 variant and has been used as a marker to identify active centromeres and DNA sequences associated with functional centromere/kinetochore complexes. In this study, up to four distinct CENH3 (BrCENH3) cDNAs were identified in individuals of each of three diploid species of Brassica. Comparison of the BrCENH3 cDNAs implied three related gene families: BrCENH3-A in Brassica rapa (AA), BrCENH3-B in B. nigra (BB), and BrCENH3-C in B. oleracea (CC). Each family encoded a histone fold domain and N-terminal histone tails that vary in length in all three families. The BrCENH3-B cDNAs have a deletion of two exons relative to BrCENH3-A and BrCENH3-C, consistent with the more ancient divergence of the BB genome. Chromatin immunoprecipitation and immunolabeling tests with anti-BrCENH3 antibodies indicated that both centromeric tandem repeats and the centromere-specific retrotransposons of Brassica are directly associated with BrCENH3 proteins. In three allotetraploid species, we find either co-transcription of the BrCENH3 genes of the ancestral diploid species or gene suppression of the BrCENH3 from one ancestor. Although B genome centromeres are occupied by BrCENH3-B in the ancestral species B. nigra, in allotetraploids both BrCENH3-A and BrCENH3-C proteins appear to assemble at these centromeres.
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