文献类型： 外文期刊
作者： Wang, Q. 1 ; Zou, D. 1 ; Ma, H. 2 ; Ji, Y. 2 ; Wang, X. 3 ;
作者机构： 1.Harbin Inst Technol, Dept Environm Engn, Harbin 150090, Heilongjiang Pr, Peoples R China
2.Univ Sci & Technol Beijing, Dept Environm Engn, Beijing 100083, Peoples R China
3.Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Cellulose;simultaneous saccharification and fermentation (SSF);lactic acid;corn straw;Trichoderma koningii
期刊名称：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY （ 影响因子：1.582； 五年影响因子：2.203 ）
ISSN： 0352-9568
年卷期： 2010 年 24 卷 3 期
页码：
收录情况： SCI
摘要： The utilization of corn straw to produce lactic acid could largely save the resource as well as decrease the amount of agricultural waste. In this study, orthogonal experimental design was adopted to investigate the optimum conditions for cellulase production from corn straw, and then the cellulase was further used to produce lactic acid through simultaneous saccharification and fermentation (SSF) process. (NH(4))(2)SO(4) and Tween 80 were also used to test their effects on lactic acid production. The optimum conditions were determined as follows: solid-to-liquid ratio was zeta = 1 : 3, pH of the substrate mixture was 4.5, inoculum size of Trichoderma koningii was G = 12 % (v/m), fermentation time was t = 84 h. Corresponding maximum cellulase of H = 2630.3 U g(-1) straw was achieved. With the produced cellulase added, the maximum lactic acid concentration of gamma = 20.2 g L(-1) could be achieved inoculated with lactic acid bacteria T50 at theta = 45 degrees C. As compared with control (non-addition), addition of w = 2% (NH(4))(2)SO(4) could increase the lactic acid by 3.6 %, while addition of Tween 80 could shorten the fermentation time from t = 108 h to t = 72 h. The result indicated that utilization of corn straw as substrate to produce cellulase and lactic acid was applicable and could reduce pollution, thus this was worth investigating further.
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