Molecular tagging and mapping of a major linked marker for taproot thickness in a Brassica crop
文献类型： 外文期刊
作者： Cao, J. S. 1 ; Lu, G. 1 ; Jiang, L. J. 2 ; Chen, L. 1 ; Xiang, X. 1 ; Chen, H. 3 ;
作者机构： 1.Zhejiang Univ, Dept Hort, Hangzhou 310029, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing 100089, Peoples R China
3.Inst Natl Vegetable Sci, Beijing 100097, Peoples R China
期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）
ISSN： 1462-0316
年卷期： 2009 年 84 卷 2 期
页码：
收录情况： SCI
摘要： A DNA marker linked to the locus controlling storage-root thickness was identified using bulked segregant analysis (BSA) in an F(2) population derived from a cross between turnip (Brassica rapa L. ssp. rapifera Matzg.) and rapeseed (Brassica rapa L.). Root-thickness in the F(2) plants showed a continuous distribution from 2.2 - 10.7 cm, suggesting the effects of a few major and minor genes. BSA, using three bulks based on root-thickness, suggested that an AFLP marker (E(ACC)M(CAG)-220) which appeared only in plants with a thickened root, was linked to a locus that controlled root size. This AFLP band was cloned, sequenced, and successfully converted into a simple, dominant, sequence characterised amplified region (SCAR) marker. This marker may help to identify turnip genotypes with thicker taproots in segregating progenies of root crops during marker-assisted selection (MAS) breeding programmes.
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